An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass

Background 1,3-Propanodiol (1,3-PD), is used in the production of polytrimethylene terephthalate (PTT), an aromatic polyester that exhibits high elastic recoveries. It is also employed as a supplement with low solidification properties, a solvent and a lubricant in the formof propylene glycol. 1,3-P...

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Autores principales:	Szymanowska-Powalowska,Daria, Leja,Katarzyna
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	C. butyricum HSP protein Microfiltration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200003
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spelling	oai:scielo:S0717-345820140002000032014-09-02An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomassSzymanowska-Powalowska,DariaLeja,Katarzyna C. butyricum HSP protein Microfiltration Background 1,3-Propanodiol (1,3-PD), is used in the production of polytrimethylene terephthalate (PTT), an aromatic polyester that exhibits high elastic recoveries. It is also employed as a supplement with low solidification properties, a solvent and a lubricant in the formof propylene glycol. 1,3-PD is effectively synthesized by a microbiological way from crude glycerol. The main problem of this technology is using a high concentration of glycerol, which is a limiting factor for bacteria cells growth (especially in batch fermentation). Results In this work, the influence of different glycerol concentration in batch fermentation on Clostridium butyricum DSP1 metabolism was investigated. The biomass was concentrated for two times with the use of membrane module (in case of increasing kinetic parameters). Increased optical density of bacteria cells six times increased the productivity of 1,3-PD in cultivation with 20 g/L of glycerol at the beginning of the process, and more than two times in cultivation with 60-80 g/L. Also the possibility of complete attenuation of 140 g/L of crude glycerol in the batch fermentation was investigated. During the cultivation, changes of protein profiles were analyzed. The most significant changes were observed in the cultivation in the medium supplemented with 80 g/L of glycerol. They related mainly to the DNA protein reconstructive systems, protective proteins (HSP), and also the enzymatic catalysts connected with glycerol metabolic pathway. Conclusions The application of filtration module in batch fermentation of crude glycerol by C. butyricum DSP1 significantly increased the productivity of the process.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.2 20142014-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200003en10.1016/j.ejbt.2013.12.010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	C. butyricum HSP protein Microfiltration
spellingShingle	C. butyricum HSP protein Microfiltration Szymanowska-Powalowska,Daria Leja,Katarzyna An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
description	Background 1,3-Propanodiol (1,3-PD), is used in the production of polytrimethylene terephthalate (PTT), an aromatic polyester that exhibits high elastic recoveries. It is also employed as a supplement with low solidification properties, a solvent and a lubricant in the formof propylene glycol. 1,3-PD is effectively synthesized by a microbiological way from crude glycerol. The main problem of this technology is using a high concentration of glycerol, which is a limiting factor for bacteria cells growth (especially in batch fermentation). Results In this work, the influence of different glycerol concentration in batch fermentation on Clostridium butyricum DSP1 metabolism was investigated. The biomass was concentrated for two times with the use of membrane module (in case of increasing kinetic parameters). Increased optical density of bacteria cells six times increased the productivity of 1,3-PD in cultivation with 20 g/L of glycerol at the beginning of the process, and more than two times in cultivation with 60-80 g/L. Also the possibility of complete attenuation of 140 g/L of crude glycerol in the batch fermentation was investigated. During the cultivation, changes of protein profiles were analyzed. The most significant changes were observed in the cultivation in the medium supplemented with 80 g/L of glycerol. They related mainly to the DNA protein reconstructive systems, protective proteins (HSP), and also the enzymatic catalysts connected with glycerol metabolic pathway. Conclusions The application of filtration module in batch fermentation of crude glycerol by C. butyricum DSP1 significantly increased the productivity of the process.
author	Szymanowska-Powalowska,Daria Leja,Katarzyna
author_facet	Szymanowska-Powalowska,Daria Leja,Katarzyna
author_sort	Szymanowska-Powalowska,Daria
title	An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
title_short	An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
title_full	An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
title_fullStr	An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
title_full_unstemmed	An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
title_sort	increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200003
work_keys_str_mv	AT szymanowskapowalowskadaria anincreasingoftheefficiencyofmicrobiologicalsynthesisof13propanediolfromcrudeglycerolbytheconcentrationofbiomass AT lejakatarzyna anincreasingoftheefficiencyofmicrobiologicalsynthesisof13propanediolfromcrudeglycerolbytheconcentrationofbiomass AT szymanowskapowalowskadaria increasingoftheefficiencyofmicrobiologicalsynthesisof13propanediolfromcrudeglycerolbytheconcentrationofbiomass AT lejakatarzyna increasingoftheefficiencyofmicrobiologicalsynthesisof13propanediolfromcrudeglycerolbytheconcentrationofbiomass
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An increasing of the efficiency of microbiological synthesis of 1,3-propanediol from crude glycerol by the concentration of biomass

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