Callus culture development of two varieties of Tagetes erecta and carotenoid production

Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poul...

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Autores principales:	Benítez-García,Israel, Vanegas-Espinoza,Pablo Emilio, Meléndez-Martínez,Antonio J, Heredia,Francisco J, Paredes-López,Octavio, Del Villar-Martínez,Alma Angélica
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	2,4-D; BA Cell development Lutein Marigold
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001
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spelling	oai:scielo:S0717-345820140003000012014-08-18Callus culture development of two varieties of Tagetes erecta and carotenoid productionBenítez-García,IsraelVanegas-Espinoza,Pablo EmilioMeléndez-Martínez,Antonio JHeredia,Francisco JParedes-López,OctavioDel Villar-Martínez,Alma Angélica 2,4-D; BA Cell development Lutein Marigold Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its production is destined mainly for ornamental purposes. Results Friable callus of T. erecta yellow flower (YF) and white flower (WF) varieties was induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 9.0 µM 4-dichlorophenoxyacetic acid (2,4-D) and 8.8 µM benzyladenine (BA). Calluses developed from both varieties were different in pigmentation. Extract characterization from callus cultures was carried out by high-performance liquid chromatography (HPLC). This analytical process detected several carotenoids; the main pigments in extracts from YF callus were lutein and zeaxanthin, whereas in the extracts of the WF callus the main pigments were lutein, zeaxanthin, β-cryptoxanthin and β-carotene. Callus cultures of T. erecta accumulated pigments even after several rounds of subculture. Conclusions WF callus appeared to be a suitable candidate as a source of different carotenoids, and tested varieties could represent an alternative for further studies about in vitro pigment production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.3 20142014-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001en10.1016/j.ejbt.2014.01.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	2,4-D; BA Cell development Lutein Marigold
spellingShingle	2,4-D; BA Cell development Lutein Marigold Benítez-García,Israel Vanegas-Espinoza,Pablo Emilio Meléndez-Martínez,Antonio J Heredia,Francisco J Paredes-López,Octavio Del Villar-Martínez,Alma Angélica Callus culture development of two varieties of Tagetes erecta and carotenoid production
description	Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its production is destined mainly for ornamental purposes. Results Friable callus of T. erecta yellow flower (YF) and white flower (WF) varieties was induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 9.0 µM 4-dichlorophenoxyacetic acid (2,4-D) and 8.8 µM benzyladenine (BA). Calluses developed from both varieties were different in pigmentation. Extract characterization from callus cultures was carried out by high-performance liquid chromatography (HPLC). This analytical process detected several carotenoids; the main pigments in extracts from YF callus were lutein and zeaxanthin, whereas in the extracts of the WF callus the main pigments were lutein, zeaxanthin, β-cryptoxanthin and β-carotene. Callus cultures of T. erecta accumulated pigments even after several rounds of subculture. Conclusions WF callus appeared to be a suitable candidate as a source of different carotenoids, and tested varieties could represent an alternative for further studies about in vitro pigment production.
author	Benítez-García,Israel Vanegas-Espinoza,Pablo Emilio Meléndez-Martínez,Antonio J Heredia,Francisco J Paredes-López,Octavio Del Villar-Martínez,Alma Angélica
author_facet	Benítez-García,Israel Vanegas-Espinoza,Pablo Emilio Meléndez-Martínez,Antonio J Heredia,Francisco J Paredes-López,Octavio Del Villar-Martínez,Alma Angélica
author_sort	Benítez-García,Israel
title	Callus culture development of two varieties of Tagetes erecta and carotenoid production
title_short	Callus culture development of two varieties of Tagetes erecta and carotenoid production
title_full	Callus culture development of two varieties of Tagetes erecta and carotenoid production
title_fullStr	Callus culture development of two varieties of Tagetes erecta and carotenoid production
title_full_unstemmed	Callus culture development of two varieties of Tagetes erecta and carotenoid production
title_sort	callus culture development of two varieties of tagetes erecta and carotenoid production
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001
work_keys_str_mv	AT benitezgarciaisrael callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction AT vanegasespinozapabloemilio callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction AT melendezmartinezantonioj callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction AT herediafranciscoj callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction AT paredeslopezoctavio callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction AT delvillarmartinezalmaangelica callusculturedevelopmentoftwovarietiesoftageteserectaandcarotenoidproduction
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Callus culture development of two varieties of Tagetes erecta and carotenoid production

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