Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design

Background In the industrial biotechnology, ligninolytic enzymes are produced by single fungal strains. Experimental evidence suggests that co-culture of ligninolytic fungi and filamentous microfungi results in an increase laccase activity. In this topic, only the ascomycete Trichoderma spp. has bee...

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Autores principales:	Chan Cupul,Wilberth, Heredia Abarca,Gabriela, Martínez Carrera,Daniel, Rodríguez Vázquez,Refugio
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Enzyme activity enhance Fungal interaction Paecilomyces carneus Time inoculation White-rot fungi
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300002
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spelling	oai:scielo:S0717-345820140003000022014-08-18Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental designChan Cupul,WilberthHeredia Abarca,GabrielaMartínez Carrera,DanielRodríguez Vázquez,Refugio Enzyme activity enhance Fungal interaction Paecilomyces carneus Time inoculation White-rot fungi Background In the industrial biotechnology, ligninolytic enzymes are produced by single fungal strains. Experimental evidence suggests that co-culture of ligninolytic fungi and filamentous microfungi results in an increase laccase activity. In this topic, only the ascomycete Trichoderma spp. has been studied broadly. However, fungal ligninolytic-filamentous microfungi biodiversity interaction in nature is abundant and poorly studied. The enhancement of laccase and manganese peroxidase (MnP) activities of Trametes maxima as a function of time inoculation of Paecilomyces carneus and under several culture conditions using Plackett-Burman experimental design (PBED) were investigated. Results The highest increases of laccase (12,382.5 U/mg protein) and MnP (564.1 U/mg protein) activities were seen in co-cultures I3 and I5, respectively, both at 10 d after inoculation. This level of activity was significantly different from the enzyme activity in non-inoculated T. maxima (4881.0 U/mg protein and 291.8 U/mg protein for laccase and MnP, respectively). PBED results showed that laccase was increased (P < 0.05) by high levels of glucose, (NH4)2SO4 and MnSO4 and low levels of KH2PO4, FeSO4 and inoculum (P < 0.05). In addition, MnP activity was increased (P < 0.05) by high yeast extract, MgSO4, CaCl2 and MnSO4 concentrations. Conclusions Interaction between indigenous fungi: T. maxima-P. carneus improves laccase and MnP activities. The inoculation time of P. carneus on T. maxima plays an important role in the laccase and MnP enhancement. The nutritional requirements for enzyme improvement in a co-culture system are different from those required for a monoculture system.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.3 20142014-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300002en10.1016/j.ejbt.2014.04.007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enzyme activity enhance Fungal interaction Paecilomyces carneus Time inoculation White-rot fungi
spellingShingle	Enzyme activity enhance Fungal interaction Paecilomyces carneus Time inoculation White-rot fungi Chan Cupul,Wilberth Heredia Abarca,Gabriela Martínez Carrera,Daniel Rodríguez Vázquez,Refugio Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
description	Background In the industrial biotechnology, ligninolytic enzymes are produced by single fungal strains. Experimental evidence suggests that co-culture of ligninolytic fungi and filamentous microfungi results in an increase laccase activity. In this topic, only the ascomycete Trichoderma spp. has been studied broadly. However, fungal ligninolytic-filamentous microfungi biodiversity interaction in nature is abundant and poorly studied. The enhancement of laccase and manganese peroxidase (MnP) activities of Trametes maxima as a function of time inoculation of Paecilomyces carneus and under several culture conditions using Plackett-Burman experimental design (PBED) were investigated. Results The highest increases of laccase (12,382.5 U/mg protein) and MnP (564.1 U/mg protein) activities were seen in co-cultures I3 and I5, respectively, both at 10 d after inoculation. This level of activity was significantly different from the enzyme activity in non-inoculated T. maxima (4881.0 U/mg protein and 291.8 U/mg protein for laccase and MnP, respectively). PBED results showed that laccase was increased (P < 0.05) by high levels of glucose, (NH4)2SO4 and MnSO4 and low levels of KH2PO4, FeSO4 and inoculum (P < 0.05). In addition, MnP activity was increased (P < 0.05) by high yeast extract, MgSO4, CaCl2 and MnSO4 concentrations. Conclusions Interaction between indigenous fungi: T. maxima-P. carneus improves laccase and MnP activities. The inoculation time of P. carneus on T. maxima plays an important role in the laccase and MnP enhancement. The nutritional requirements for enzyme improvement in a co-culture system are different from those required for a monoculture system.
author	Chan Cupul,Wilberth Heredia Abarca,Gabriela Martínez Carrera,Daniel Rodríguez Vázquez,Refugio
author_facet	Chan Cupul,Wilberth Heredia Abarca,Gabriela Martínez Carrera,Daniel Rodríguez Vázquez,Refugio
author_sort	Chan Cupul,Wilberth
title	Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
title_short	Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
title_full	Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
title_fullStr	Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
title_full_unstemmed	Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
title_sort	enhancement of ligninolytic enzyme activities in a trametes maxima-paecilomyces carneus co-culture: key factors revealed after screening using a plackett-burman experimental design
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300002
work_keys_str_mv	AT chancupulwilberth enhancementofligninolyticenzymeactivitiesinatrametesmaximapaecilomycescarneuscoculturekeyfactorsrevealedafterscreeningusingaplackettburmanexperimentaldesign AT herediaabarcagabriela enhancementofligninolyticenzymeactivitiesinatrametesmaximapaecilomycescarneuscoculturekeyfactorsrevealedafterscreeningusingaplackettburmanexperimentaldesign AT martinezcarreradaniel enhancementofligninolyticenzymeactivitiesinatrametesmaximapaecilomycescarneuscoculturekeyfactorsrevealedafterscreeningusingaplackettburmanexperimentaldesign AT rodriguezvazquezrefugio enhancementofligninolyticenzymeactivitiesinatrametesmaximapaecilomycescarneuscoculturekeyfactorsrevealedafterscreeningusingaplackettburmanexperimentaldesign
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Enhancement of ligninolytic enzyme activities in a Trametes maxima-Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design

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