Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle

Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In...

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Autores principales:	Fu,Changzhen, Wang,Hong, Li,Yaokun, Wang,Jiali, Cheng,Gong, Wang,Hongbao, Yang,Wucai, Zan,Linsen
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004
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spelling	oai:scielo:S0717-345820140004000042014-09-02Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattleFu,ChangzhenWang,HongLi,YaokunWang,JialiCheng,GongWang,HongbaoYang,WucaiZan,Linsen Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study. g.244T>C located at 5'untranslated region (5'UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA. Conclusions Analysis results showed that these three SNPs were significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs). We suggested that CDIPT gene may have potential effects on BMTs and MQTs and can be used for marker-assisted selection.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.4 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004en10.1016/j.ejbt.2014.04.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP
spellingShingle	Body measurement traits Gene expression Meat quality traits PCR-RFLP SNP Fu,Changzhen Wang,Hong Li,Yaokun Wang,Jiali Cheng,Gong Wang,Hongbao Yang,Wucai Zan,Linsen Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
description	Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality traits of Qinchuan cattle. Blood samples were obtained from 638 Qinchuan cattle aged from 18 to 24 months. DNA sequencing and PCR-restriction fragment length polymorphism (RFLP) were used to find CDIPT gene single nucleotide polymorphism (SNP). Three SNPs g.244T>C (NCBI: rs42069760), g.1496G>A and g.1514G>A were found in this study. g.244T>C located at 5'untranslated region (5'UTR) of exon 1 showed three genotypes: TT, TC and CC. g.1496G>A and g.1514G>A detected the first time were located in intron 3 and showed the same genotypes: GG, GA and AA. Conclusions Analysis results showed that these three SNPs were significantly associated with body measurement traits (BMTs) and meat quality traits (MQTs). We suggested that CDIPT gene may have potential effects on BMTs and MQTs and can be used for marker-assisted selection.
author	Fu,Changzhen Wang,Hong Li,Yaokun Wang,Jiali Cheng,Gong Wang,Hongbao Yang,Wucai Zan,Linsen
author_facet	Fu,Changzhen Wang,Hong Li,Yaokun Wang,Jiali Cheng,Gong Wang,Hongbao Yang,Wucai Zan,Linsen
author_sort	Fu,Changzhen
title	Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
title_short	Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
title_full	Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
title_fullStr	Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
title_full_unstemmed	Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle
title_sort	expression profiles and polymorphism analysis of cdipt gene on qinchuan cattle
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004
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_version_	1718441893155569664

Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle

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