Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus

Background Lactic acid bacteria are able to reduce the immunoreactivity of proteins of cereal grains during wheat dough fermentation or may be a source of proteolytic preparations added during bread making. The key enzyme in prolamin degradation is prolyl endopeptidase. This study was aimed at optim...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Brzozowski,Bartosz, Lewandowska,Malgorzata
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Central composite design Plackett-Burman design Response surface methodology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582014000500003
record_format	dspace
spelling	oai:scielo:S0717-345820140005000032014-10-07Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilusBrzozowski,BartoszLewandowska,Malgorzata Central composite design Plackett-Burman design Response surface methodology Background Lactic acid bacteria are able to reduce the immunoreactivity of proteins of cereal grains during wheat dough fermentation or may be a source of proteolytic preparations added during bread making. The key enzyme in prolamin degradation is prolyl endopeptidase. This study was aimed at optimizing the composition of a culture medium and culture conditions that would enhance the synthesis of intracellular prolyl endopeptidase (PEP) by Lactobacillus acidophilus 5e2. Results The application of Plackett-Burman screening plans enabled demonstrating that the concentration of a nitrogen source in the culture and the initial pH value of the culture medium were significant for PEP synthesis. Further optimization conducted with the method of central composite designs (CCD) confirmed both the linear and square impact of nitrogen concentration and initial pH value of the culture medium on PEP production. In turn, the response surface method (RSM) allowed determining the optimal nitrogen concentration and pH value at 26.88 g/l and pH 4.85, respectively. Conclusions Validation of the resultant model enabled over 3-fold increase in the quantity of the synthesized enzyme.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.5 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500003en10.1016/j.ejbt.2014.07.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Central composite design Plackett-Burman design Response surface methodology
spellingShingle	Central composite design Plackett-Burman design Response surface methodology Brzozowski,Bartosz Lewandowska,Malgorzata Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
description	Background Lactic acid bacteria are able to reduce the immunoreactivity of proteins of cereal grains during wheat dough fermentation or may be a source of proteolytic preparations added during bread making. The key enzyme in prolamin degradation is prolyl endopeptidase. This study was aimed at optimizing the composition of a culture medium and culture conditions that would enhance the synthesis of intracellular prolyl endopeptidase (PEP) by Lactobacillus acidophilus 5e2. Results The application of Plackett-Burman screening plans enabled demonstrating that the concentration of a nitrogen source in the culture and the initial pH value of the culture medium were significant for PEP synthesis. Further optimization conducted with the method of central composite designs (CCD) confirmed both the linear and square impact of nitrogen concentration and initial pH value of the culture medium on PEP production. In turn, the response surface method (RSM) allowed determining the optimal nitrogen concentration and pH value at 26.88 g/l and pH 4.85, respectively. Conclusions Validation of the resultant model enabled over 3-fold increase in the quantity of the synthesized enzyme.
author	Brzozowski,Bartosz Lewandowska,Malgorzata
author_facet	Brzozowski,Bartosz Lewandowska,Malgorzata
author_sort	Brzozowski,Bartosz
title	Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
title_short	Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
title_full	Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
title_fullStr	Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
title_full_unstemmed	Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus
title_sort	prolyl endopeptidase - optimization of medium and culture conditions for enhanced production by lactobacillus acidophilus
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500003
work_keys_str_mv	AT brzozowskibartosz prolylendopeptidaseoptimizationofmediumandcultureconditionsforenhancedproductionbylactobacillusacidophilus AT lewandowskamalgorzata prolylendopeptidaseoptimizationofmediumandcultureconditionsforenhancedproductionbylactobacillusacidophilus
_version_	1718441895162544128

Prolyl endopeptidase - Optimization of medium and culture conditions for enhanced production by Lactobacillus acidophilus

Ejemplares similares