Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts
Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2014
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582014000500006 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820140005000062014-10-07Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblastsLi,XinxinWang,JiwenLiu,HeheWang,HaohanSun,LingliYang,ChaoLi,LiangZheng,Yi Follistatin Muscle hypertrophy Overexpression Transfection efficiency Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in these cells significantly inhibited the mRNA expression of MSTN (a target gene that is negatively regulated by FST). Conclusions The duck pEGFP-FST vector has been constructed successfully and exhibits biological activity by promoting myoblast proliferation and differentiation in vitro.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.5 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006en10.1016/j.ejbt.2014.07.002 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Follistatin Muscle hypertrophy Overexpression Transfection efficiency |
| spellingShingle |
Follistatin Muscle hypertrophy Overexpression Transfection efficiency Li,Xinxin Wang,Jiwen Liu,Hehe Wang,Haohan Sun,Lingli Yang,Chao Li,Liang Zheng,Yi Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| description |
Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in these cells significantly inhibited the mRNA expression of MSTN (a target gene that is negatively regulated by FST). Conclusions The duck pEGFP-FST vector has been constructed successfully and exhibits biological activity by promoting myoblast proliferation and differentiation in vitro. |
| author |
Li,Xinxin Wang,Jiwen Liu,Hehe Wang,Haohan Sun,Lingli Yang,Chao Li,Liang Zheng,Yi |
| author_facet |
Li,Xinxin Wang,Jiwen Liu,Hehe Wang,Haohan Sun,Lingli Yang,Chao Li,Liang Zheng,Yi |
| author_sort |
Li,Xinxin |
| title |
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| title_short |
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| title_full |
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| title_fullStr |
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| title_full_unstemmed |
Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts |
| title_sort |
construction of a eukaryotic expression vector for pegfp-fst and its biological activity in duck myoblasts |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2014 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 |
| work_keys_str_mv |
AT lixinxin constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT wangjiwen constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT liuhehe constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT wanghaohan constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT sunlingli constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT yangchao constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT liliang constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts AT zhengyi constructionofaeukaryoticexpressionvectorforpegfpfstanditsbiologicalactivityinduckmyoblasts |
| _version_ |
1718441896012939264 |