Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles betw...

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Autores principales:	Yu,Tao, Wang,Zhenhua, Jin,Xiaochun, Liu,Xianjun, Kan,Shuaishuai
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Differentially expressed genes Head smut resistance gene Maize Microarray
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500007
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spelling	oai:scielo:S0717-345820140005000072014-10-07Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)Yu,TaoWang,ZhenhuaJin,XiaochunLiu,XianjunKan,Shuaishuai Differentially expressed genes Head smut resistance gene Maize Microarray Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles between a disease resistant near isogenic line (NIL) and a highly susceptible inbred line after S. reilianum was injected with an artificial inoculation method. Results Levels of expression for 3,532 genes accounting for 23.8% of the total probes changed after inoculation. Gene Ontology analysis revealed that the differentially expressed genes participated in physiological and biochemical pathways. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that plant-pathogen interaction, natural killer cell mediated cytotoxicity and benzoxazinoid biosynthesis pathways play important roles in resistance to head smut. Three head smut resistance-related candidate genes, CLAVATA1, bassinosteroid insensitive 1-associated receptor kinase 1 and LOC100217307 with leucine-rich repeat (LRR) conserved domains were identified, each of which is in maize mapping bin 2.09, a region previously shown to include a major QTL for head smut resistance. Furthermore, LOC100217307 was validated by quantitative real-time (qRT)-PCR inferring that this gene may be involved in the resistance to head smut of maize. Conclusions This study provided valuable information for cloning, functional analysis and marker assisted breeding of head smut resistance genes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.5 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500007en10.1016/j.ejbt.2014.07.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Differentially expressed genes Head smut resistance gene Maize Microarray
spellingShingle	Differentially expressed genes Head smut resistance gene Maize Microarray Yu,Tao Wang,Zhenhua Jin,Xiaochun Liu,Xianjun Kan,Shuaishuai Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
description	Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles between a disease resistant near isogenic line (NIL) and a highly susceptible inbred line after S. reilianum was injected with an artificial inoculation method. Results Levels of expression for 3,532 genes accounting for 23.8% of the total probes changed after inoculation. Gene Ontology analysis revealed that the differentially expressed genes participated in physiological and biochemical pathways. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that plant-pathogen interaction, natural killer cell mediated cytotoxicity and benzoxazinoid biosynthesis pathways play important roles in resistance to head smut. Three head smut resistance-related candidate genes, CLAVATA1, bassinosteroid insensitive 1-associated receptor kinase 1 and LOC100217307 with leucine-rich repeat (LRR) conserved domains were identified, each of which is in maize mapping bin 2.09, a region previously shown to include a major QTL for head smut resistance. Furthermore, LOC100217307 was validated by quantitative real-time (qRT)-PCR inferring that this gene may be involved in the resistance to head smut of maize. Conclusions This study provided valuable information for cloning, functional analysis and marker assisted breeding of head smut resistance genes.
author	Yu,Tao Wang,Zhenhua Jin,Xiaochun Liu,Xianjun Kan,Shuaishuai
author_facet	Yu,Tao Wang,Zhenhua Jin,Xiaochun Liu,Xianjun Kan,Shuaishuai
author_sort	Yu,Tao
title	Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
title_short	Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
title_full	Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
title_fullStr	Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
title_full_unstemmed	Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)
title_sort	analysis of gene expression profiles in response to sporisorium reilianum f. sp. zeae in maize (zea mays l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500007
work_keys_str_mv	AT yutao analysisofgeneexpressionprofilesinresponsetosporisoriumreilianumfspzeaeinmaizezeamaysl AT wangzhenhua analysisofgeneexpressionprofilesinresponsetosporisoriumreilianumfspzeaeinmaizezeamaysl AT jinxiaochun analysisofgeneexpressionprofilesinresponsetosporisoriumreilianumfspzeaeinmaizezeamaysl AT liuxianjun analysisofgeneexpressionprofilesinresponsetosporisoriumreilianumfspzeaeinmaizezeamaysl AT kanshuaishuai analysisofgeneexpressionprofilesinresponsetosporisoriumreilianumfspzeaeinmaizezeamaysl
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Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

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