On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae

Background The selection of new yeast strains could lead to improvements in bioethanol production. Here, we have studied the fermentative capacity of different auxotrophic mutants of Saccharomyces cerevisiae, which are routinely used as hosts for the production of heterologous proteins. It has recen...

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Autores principales:	Paciello,Lucia, Zueco,Jesus, Landi,Carmine
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Auxotrophic yeast CEN.PK strains Ethanol yields Fermentative capacity Fermentative metabolism
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500009
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spelling	oai:scielo:S0717-345820140005000092014-10-07On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiaePaciello,LuciaZueco,JesusLandi,Carmine Auxotrophic yeast CEN.PK strains Ethanol yields Fermentative capacity Fermentative metabolism Background The selection of new yeast strains could lead to improvements in bioethanol production. Here, we have studied the fermentative capacity of different auxotrophic mutants of Saccharomyces cerevisiae, which are routinely used as hosts for the production of heterologous proteins. It has recently been found that these strains exhibit physiological alterations and peculiar sensitivities with respect to the parental prototrophic strains from which they derive. In this work the performance of auxotrophic S. cerevisiae CEN.PK strains was compared to the corresponding prototrophic strain, to S. cerevisiae T5bV, a strain isolated from grape must and to another auxotrophic strain, S. cerevisiae BY4741. Results The results indicate that the fermentative capacity of strains grown in 2% glucose was similar in all the strains tested. However, in 15% initial glucose, the auxotrophic strains exhibited a more than doubled ethanol yield on biomass (10 g g- 1dw) compared to the prototrophic strains (less than 5 g g- 1dw). Other tests have also evidenced that in medium depletion conditions, ethanol production continues after growth arrest. Conclusions The results highlight the capacity of auxotrophic yeast strains to produce ethanol per mass unit, in a higher amount with respect to the prototrophic ones. This leads to potential applications for auxotrophic strains of S. cerevisiae in the production of ethanol in both homogeneous and heterogeneous phases (immobilized systems). The higher ethanol yield on biomass would be advantageous in immobilized cell systems, as a reduced yeast biomass could greatly reduce the mass transfer limitations through the immobilization matrix.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.5 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500009en10.1016/j.ejbt.2014.07.007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Auxotrophic yeast CEN.PK strains Ethanol yields Fermentative capacity Fermentative metabolism
spellingShingle	Auxotrophic yeast CEN.PK strains Ethanol yields Fermentative capacity Fermentative metabolism Paciello,Lucia Zueco,Jesus Landi,Carmine On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
description	Background The selection of new yeast strains could lead to improvements in bioethanol production. Here, we have studied the fermentative capacity of different auxotrophic mutants of Saccharomyces cerevisiae, which are routinely used as hosts for the production of heterologous proteins. It has recently been found that these strains exhibit physiological alterations and peculiar sensitivities with respect to the parental prototrophic strains from which they derive. In this work the performance of auxotrophic S. cerevisiae CEN.PK strains was compared to the corresponding prototrophic strain, to S. cerevisiae T5bV, a strain isolated from grape must and to another auxotrophic strain, S. cerevisiae BY4741. Results The results indicate that the fermentative capacity of strains grown in 2% glucose was similar in all the strains tested. However, in 15% initial glucose, the auxotrophic strains exhibited a more than doubled ethanol yield on biomass (10 g g- 1dw) compared to the prototrophic strains (less than 5 g g- 1dw). Other tests have also evidenced that in medium depletion conditions, ethanol production continues after growth arrest. Conclusions The results highlight the capacity of auxotrophic yeast strains to produce ethanol per mass unit, in a higher amount with respect to the prototrophic ones. This leads to potential applications for auxotrophic strains of S. cerevisiae in the production of ethanol in both homogeneous and heterogeneous phases (immobilized systems). The higher ethanol yield on biomass would be advantageous in immobilized cell systems, as a reduced yeast biomass could greatly reduce the mass transfer limitations through the immobilization matrix.
author	Paciello,Lucia Zueco,Jesus Landi,Carmine
author_facet	Paciello,Lucia Zueco,Jesus Landi,Carmine
author_sort	Paciello,Lucia
title	On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
title_short	On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
title_full	On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
title_fullStr	On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
title_full_unstemmed	On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
title_sort	on the fermentative behavior of auxotrophic strains of saccharomyces cerevisiae
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500009
work_keys_str_mv	AT paciellolucia onthefermentativebehaviorofauxotrophicstrainsofsaccharomycescerevisiae AT zuecojesus onthefermentativebehaviorofauxotrophicstrainsofsaccharomycescerevisiae AT landicarmine onthefermentativebehaviorofauxotrophicstrainsofsaccharomycescerevisiae
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On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae

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