Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation

Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and...

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Autores principales:	Ding,Fang, Yuan,Xin, Li,Qingqing, Sun,Wenqiang, Gan,Chao, He,Hua, Song,Chenling, Wang,Jiwen
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Expression pattern FAS Oleic acid Peking duck
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600001
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spelling	oai:scielo:S0717-345820140006000012014-12-10Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiationDing,FangYuan,XinLi,QingqingSun,WenqiangGan,ChaoHe,HuaSong,ChenlingWang,Jiwen Expression pattern FAS Oleic acid Peking duck Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 µM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05). Conclusion We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.6 20142014-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600001en10.1016/j.ejbt.2014.08.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Expression pattern FAS Oleic acid Peking duck
spellingShingle	Expression pattern FAS Oleic acid Peking duck Ding,Fang Yuan,Xin Li,Qingqing Sun,Wenqiang Gan,Chao He,Hua Song,Chenling Wang,Jiwen Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
description	Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and investigate its expression during adipocyte differentiation. Results We have isolated a 7654 bp fragment from Peking duck adipocytes that corresponds to the FAS gene. The cloned fragment contains an open reading frame of 7545 bp, encodes a 2515 amino acid protein, and displays high nucleotide and amino acid homology to avian FAS orthologs. Twelve hour treatment of oleic acid significantly up-regulated the expression of FAS in duck preadipocytes (P < 0.05). However, 1000 µM treatment of oleic acid exhibited lipotoxic effect on cell viability (P < 0.05). In addition, during the first 24 h of duck adipocyte differentiation FAS was induced; however, after 24 h its expression level declined (P < 0.05). Conclusion We have successfully cloned and characterized Peking duck FAS. FAS was induced during adipocyte differentiation and by oleic acid treatment. These findings suggest that Peking duck FAS plays a similar role to mammalian FAS during adipocyte differentiation.
author	Ding,Fang Yuan,Xin Li,Qingqing Sun,Wenqiang Gan,Chao He,Hua Song,Chenling Wang,Jiwen
author_facet	Ding,Fang Yuan,Xin Li,Qingqing Sun,Wenqiang Gan,Chao He,Hua Song,Chenling Wang,Jiwen
author_sort	Ding,Fang
title	Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
title_short	Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
title_full	Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
title_fullStr	Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
title_full_unstemmed	Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation
title_sort	cloning, characterization and expression of peking duck fatty acid synthase during adipocyte differentiation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600001
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Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation

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