Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production

Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cel...

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Autores principales:	Yang,Weiping, Meng,Fanxu, Peng,Jiayin, Han,Peng, Fang,Fang, Ma,Li, Cao,Binyun
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Carboxymethyl cellulose Cellulolytic bacterium Fermentation conditions Screening and identification Tibetan pig
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002
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spelling	oai:scielo:S0717-345820140006000022014-12-10Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase productionYang,WeipingMeng,FanxuPeng,JiayinHan,PengFang,FangMa,LiCao,Binyun Carboxymethyl cellulose Cellulolytic bacterium Fermentation conditions Screening and identification Tibetan pig Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24-48 h. Conclusions The isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.6 20142014-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002en10.1016/j.ejbt.2014.08.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Carboxymethyl cellulose Cellulolytic bacterium Fermentation conditions Screening and identification Tibetan pig
spellingShingle	Carboxymethyl cellulose Cellulolytic bacterium Fermentation conditions Screening and identification Tibetan pig Yang,Weiping Meng,Fanxu Peng,Jiayin Han,Peng Fang,Fang Ma,Li Cao,Binyun Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
description	Background The Tibetan pig is a pig breed with excellent grazing characteristics indigenous to the Qinghai-Tibet plateau in China. Under conditions of barn feeding, 90% of its diet consists of forage grass, which helps meet its nutritional needs. The present study aimed to isolate and identify a cellulolytic bacterium from the Tibetan pig's intestine and investigate cellulase production by this bacterium. The study purpose is to provide a basic theory for the research and development of herbivore characteristics and to identify a source of probiotics from the Tibetan pig. Results A cellulolytic bacterium was isolated from a Tibetan pig's intestine and identified based on morphological, physiological, and biochemical characteristics as well as 16S rRNA analysis; it was designated Bacillus subtilis BY-2. Examination of its growth characteristics showed that its growth curve entered the logarithmic phase after 8-12 h and the stable growth phase being between 20 and 40 h. The best carbon source for fermentation was 1% corn flour, while 2% peptone and yeast powder compound were the best nitrogen sources. The initial pH during fermentation was 5.5, with 4% inoculum, resulting in a high and stable amount of enzyme in 24-48 h. Conclusions The isolated BY-2 strain rapidly grew and produced cellulase. We believe that BY-2 cellulase can help overcome the shortage of endogenous animal cellulase, improve the utilization rate of roughage, and provide strain sources for research on porcine probiotics.
author	Yang,Weiping Meng,Fanxu Peng,Jiayin Han,Peng Fang,Fang Ma,Li Cao,Binyun
author_facet	Yang,Weiping Meng,Fanxu Peng,Jiayin Han,Peng Fang,Fang Ma,Li Cao,Binyun
author_sort	Yang,Weiping
title	Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
title_short	Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
title_full	Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
title_fullStr	Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
title_full_unstemmed	Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production
title_sort	isolation and identification of a cellulolytic bacterium from the tibetan pig's intestine and investigation of its cellulase production
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600002
work_keys_str_mv	AT yangweiping isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT mengfanxu isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT pengjiayin isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT hanpeng isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT fangfang isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT mali isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction AT caobinyun isolationandidentificationofacellulolyticbacteriumfromthetibetanpigsintestineandinvestigationofitscellulaseproduction
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Isolation and identification of a cellulolytic bacterium from the Tibetan pig's intestine and investigation of its cellulase production

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