Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to ana...

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Autores principales:	Miao,Hongxia, Wang,Yuan, Liu,Juhua, Jia,Caihong, Hu,Wei, Sun,Peiguang, Jin,Zhiqiang, Xu,Biyu
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	ASR Banana Expression patterns Salt resistance
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600006
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spelling	oai:scielo:S0717-345820140006000062014-12-10Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stressMiao,HongxiaWang,YuanLiu,JuhuaJia,CaihongHu,WeiSun,PeiguangJin,ZhiqiangXu,Biyu ASR Banana Expression patterns Salt resistance Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis the sequence characterization of banana ASR1, and identify its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in Arabidopsis. The purpose was to evaluate the role of banana ASR1 to salt stress tolerance employed by plants. Results A full-length cDNA isolated from banana fruit was named MaASR1, and it had a 432 bp open reading frame (ORF) encoding 143 amino acids. MaASR1 was preferential expression in roots and leaves compared to low expression in fruits, rhizomes and flowers. Under salt stress, the expression of MaASR1 quickly increased and highest expression level was detected in roots and leaves at 4 h, and then gradually decreased. These results suggested that MaASR1 expression was induced under salt stress. MaASR1 protein was localized in the nucleus and plasma membrane. MaASR1 was transformed to Arabidopsis and verified by southern and northern analysis, transgenic lines L14 and L38 integrated one and two copies of MaASR1, respectively, while overexpression in transgenic lines provided evidence for the role of MaASR1 to salt stress tolerance. Conclusions This study demonstrated that overexpression of MaASR1 in Arabidopsis confers salt stress tolerance by reducing the expression of ABA/stress-responsive genes, but does not affect the expression of the ABA-independent pathway and biosynthesis pathway genes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.6 20142014-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600006en10.1016/j.ejbt.2014.09.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ASR Banana Expression patterns Salt resistance
spellingShingle	ASR Banana Expression patterns Salt resistance Miao,Hongxia Wang,Yuan Liu,Juhua Jia,Caihong Hu,Wei Sun,Peiguang Jin,Zhiqiang Xu,Biyu Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
description	Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis the sequence characterization of banana ASR1, and identify its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in Arabidopsis. The purpose was to evaluate the role of banana ASR1 to salt stress tolerance employed by plants. Results A full-length cDNA isolated from banana fruit was named MaASR1, and it had a 432 bp open reading frame (ORF) encoding 143 amino acids. MaASR1 was preferential expression in roots and leaves compared to low expression in fruits, rhizomes and flowers. Under salt stress, the expression of MaASR1 quickly increased and highest expression level was detected in roots and leaves at 4 h, and then gradually decreased. These results suggested that MaASR1 expression was induced under salt stress. MaASR1 protein was localized in the nucleus and plasma membrane. MaASR1 was transformed to Arabidopsis and verified by southern and northern analysis, transgenic lines L14 and L38 integrated one and two copies of MaASR1, respectively, while overexpression in transgenic lines provided evidence for the role of MaASR1 to salt stress tolerance. Conclusions This study demonstrated that overexpression of MaASR1 in Arabidopsis confers salt stress tolerance by reducing the expression of ABA/stress-responsive genes, but does not affect the expression of the ABA-independent pathway and biosynthesis pathway genes.
author	Miao,Hongxia Wang,Yuan Liu,Juhua Jia,Caihong Hu,Wei Sun,Peiguang Jin,Zhiqiang Xu,Biyu
author_facet	Miao,Hongxia Wang,Yuan Liu,Juhua Jia,Caihong Hu,Wei Sun,Peiguang Jin,Zhiqiang Xu,Biyu
author_sort	Miao,Hongxia
title	Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
title_short	Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
title_full	Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
title_fullStr	Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
title_full_unstemmed	Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress
title_sort	molecular cloning and expression analysis of the maasr1 gene in banana and functional characterization under salt stress
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600006
work_keys_str_mv	AT miaohongxia molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT wangyuan molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT liujuhua molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT jiacaihong molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT huwei molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT sunpeiguang molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT jinzhiqiang molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress AT xubiyu molecularcloningandexpressionanalysisofthemaasr1geneinbananaandfunctionalcharacterizationundersaltstress
_version_	1718441898688905216

Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

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