Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization

Background Peanut (Arachis hypogaea L.) is an important economic and oilseed crop. Long-term rainless conditions and seasonal droughts can limit peanut yields and were conducive to preharvest aflatoxin contamination. To elucidate the molecular mechanisms by which peanut responds and adapts to water...

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Autores principales:	Ding,Hong, Zhang,Zhi Meng, Qin,Fei Fei, Dai,Liang Xiang, Li,Chun Juan, Ci,Dun Wei, Song,Wen Wu
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Drought-related genes Drought stress GO and KEGG analyses Real-time reverse transcription-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600008
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spelling	oai:scielo:S0717-345820140006000082014-12-10Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridizationDing,HongZhang,Zhi MengQin,Fei FeiDai,Liang XiangLi,Chun JuanCi,Dun WeiSong,Wen Wu Drought-related genes Drought stress GO and KEGG analyses Real-time reverse transcription-PCR Background Peanut (Arachis hypogaea L.) is an important economic and oilseed crop. Long-term rainless conditions and seasonal droughts can limit peanut yields and were conducive to preharvest aflatoxin contamination. To elucidate the molecular mechanisms by which peanut responds and adapts to water limited conditions, we isolated and characterized several drought-induced genes from peanut roots using a suppression subtractive hybridization (SSH) technique. Results RNA was extracted from peanut roots subjected to a water stress treatment (45% field capacity) and from control plants (75% field capacity), and used to generate an SSH cDNA library. A total of 111 non-redundant sequences were obtained, with 80 unique transcripts showing homology to known genes and 31 clones with no similarity to either hypothetical or known proteins. GO and KEGG analyses of these differentially expressed ESTs indicated that drought-related responses in peanut could mainly be attributed to genes involved in cellular structure and metabolism. In addition, we examined the expression patterns of seven differentially expressed candidate genes using real-time reverse transcription-PCR (qRT-PCR) and confirmed that all were up-regulated in roots in response to drought stress, but to differing extents. Conclusions We successfully constructed an SSH cDNA library in peanut roots and identified several drought-related genes. Our results serve as a foundation for future studies into the elucidation of the drought stress response mechanisms of peanut.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.6 20142014-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600008en10.1016/j.ejbt.2014.09.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Drought-related genes Drought stress GO and KEGG analyses Real-time reverse transcription-PCR
spellingShingle	Drought-related genes Drought stress GO and KEGG analyses Real-time reverse transcription-PCR Ding,Hong Zhang,Zhi Meng Qin,Fei Fei Dai,Liang Xiang Li,Chun Juan Ci,Dun Wei Song,Wen Wu Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
description	Background Peanut (Arachis hypogaea L.) is an important economic and oilseed crop. Long-term rainless conditions and seasonal droughts can limit peanut yields and were conducive to preharvest aflatoxin contamination. To elucidate the molecular mechanisms by which peanut responds and adapts to water limited conditions, we isolated and characterized several drought-induced genes from peanut roots using a suppression subtractive hybridization (SSH) technique. Results RNA was extracted from peanut roots subjected to a water stress treatment (45% field capacity) and from control plants (75% field capacity), and used to generate an SSH cDNA library. A total of 111 non-redundant sequences were obtained, with 80 unique transcripts showing homology to known genes and 31 clones with no similarity to either hypothetical or known proteins. GO and KEGG analyses of these differentially expressed ESTs indicated that drought-related responses in peanut could mainly be attributed to genes involved in cellular structure and metabolism. In addition, we examined the expression patterns of seven differentially expressed candidate genes using real-time reverse transcription-PCR (qRT-PCR) and confirmed that all were up-regulated in roots in response to drought stress, but to differing extents. Conclusions We successfully constructed an SSH cDNA library in peanut roots and identified several drought-related genes. Our results serve as a foundation for future studies into the elucidation of the drought stress response mechanisms of peanut.
author	Ding,Hong Zhang,Zhi Meng Qin,Fei Fei Dai,Liang Xiang Li,Chun Juan Ci,Dun Wei Song,Wen Wu
author_facet	Ding,Hong Zhang,Zhi Meng Qin,Fei Fei Dai,Liang Xiang Li,Chun Juan Ci,Dun Wei Song,Wen Wu
author_sort	Ding,Hong
title	Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
title_short	Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
title_full	Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
title_fullStr	Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
title_full_unstemmed	Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
title_sort	isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600008
work_keys_str_mv	AT dinghong isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT zhangzhimeng isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT qinfeifei isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT dailiangxiang isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT lichunjuan isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT cidunwei isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization AT songwenwu isolationandcharacterizationofdroughtresponsivegenesfrompeanutrootsbysuppressionsubtractivehybridization
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Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization

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