1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch

Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol...

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Autor principal:	Szymanowska-Powalowska,Daria
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Environmental stress Fermentation High-performance liquid chromatography
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011
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spelling	oai:scielo:S0717-345820140006000112014-12-101,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batchSzymanowska-Powalowska,Daria Environmental stress Fermentation High-performance liquid chromatography Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum productivity, obtained during the second cycle, reached 1.68 g/L/h. Additionally, experiments conducted in parallel to the above involved using the entire quantity of the culture broth removed from the bioreactor to inoculate successive portions of fermentation media containing crude glycerol at concentrations of 80 g/L and 100 g/L. Under those conditions, the maximum 1,3-PD concentrations were 43.2 g/L and 54.2 g/L. Conclusions The experiments proved that by using a portion of metabolically active biomass as inoculum for another fermentation formula it is possible to eliminate the stage of inoculum growth and thereby reduce the length of the whole operation. Additionally, that strategy avoids the phase of microbial adaptation to a different source of carbon such as crude glycerol, which is more difficult to utilize, thus improving the kinetic parameters of 1,3-PD production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.6 20142014-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011en10.1016/j.ejbt.2014.10.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Environmental stress Fermentation High-performance liquid chromatography
spellingShingle	Environmental stress Fermentation High-performance liquid chromatography Szymanowska-Powalowska,Daria 1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
description	Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum productivity, obtained during the second cycle, reached 1.68 g/L/h. Additionally, experiments conducted in parallel to the above involved using the entire quantity of the culture broth removed from the bioreactor to inoculate successive portions of fermentation media containing crude glycerol at concentrations of 80 g/L and 100 g/L. Under those conditions, the maximum 1,3-PD concentrations were 43.2 g/L and 54.2 g/L. Conclusions The experiments proved that by using a portion of metabolically active biomass as inoculum for another fermentation formula it is possible to eliminate the stage of inoculum growth and thereby reduce the length of the whole operation. Additionally, that strategy avoids the phase of microbial adaptation to a different source of carbon such as crude glycerol, which is more difficult to utilize, thus improving the kinetic parameters of 1,3-PD production.
author	Szymanowska-Powalowska,Daria
author_facet	Szymanowska-Powalowska,Daria
author_sort	Szymanowska-Powalowska,Daria
title	1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
title_short	1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
title_full	1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
title_fullStr	1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
title_full_unstemmed	1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch
title_sort	1,3-propanediol production from crude glycerol by clostridium butyricum dsp1 in repeated batch
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011
work_keys_str_mv	AT szymanowskapowalowskadaria 13propanediolproductionfromcrudeglycerolbyclostridiumbutyricumdsp1inrepeatedbatch
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1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch

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