Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens

Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optim...

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Autores principales:	M.M. Silva,Germana, Bezerra,Raquel P, Teixeira,José A, Porto,Tatiana S, Lima-Filho,José L, Porto,Ana Lúcia F
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Actinomycetes Factorial design Glucose Microbial enzymes Soybean flour
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100004
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spelling	oai:scielo:S0717-345820150001000042015-03-09Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichensM.M. Silva,GermanaBezerra,Raquel PTeixeira,José APorto,Tatiana SLima-Filho,José LPorto,Ana Lúcia F Actinomycetes Factorial design Glucose Microbial enzymes Soybean flour Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm² was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100004en10.1016/j.ejbt.2014.11.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Actinomycetes Factorial design Glucose Microbial enzymes Soybean flour
spellingShingle	Actinomycetes Factorial design Glucose Microbial enzymes Soybean flour M.M. Silva,Germana Bezerra,Raquel P Teixeira,José A Porto,Tatiana S Lima-Filho,José L Porto,Ana Lúcia F Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
description	Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm² was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.
author	M.M. Silva,Germana Bezerra,Raquel P Teixeira,José A Porto,Tatiana S Lima-Filho,José L Porto,Ana Lúcia F
author_facet	M.M. Silva,Germana Bezerra,Raquel P Teixeira,José A Porto,Tatiana S Lima-Filho,José L Porto,Ana Lúcia F
author_sort	M.M. Silva,Germana
title	Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
title_short	Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
title_full	Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
title_fullStr	Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
title_full_unstemmed	Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
title_sort	fibrinolytic protease production by new streptomyces sp: dpua 1576 from amazon lichens
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100004
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Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens

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