A comparative proteomic study of white and black glutinous rice leaves

Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyan...

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Autores principales: Phonsakhan,Wipada, Kong-Ngern,Kanlaya
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2015
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006
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spelling oai:scielo:S0717-345820150001000062015-03-09A comparative proteomic study of white and black glutinous rice leavesPhonsakhan,WipadaKong-Ngern,Kanlaya 2D-PAGE Anthocyanin LC-MS/MS Oryza sativa L Proteomics Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5 software. The results showed that the protein profiles of these two rice cultivars were different, with at least six protein spots that were detected only in Br no. 19. In addition, seven protein spots accumulated at higher levels in Br no. 19 than in RD 6. Conclusion The protein spot S1 (AP005098.4) is homologous to the Rc protein. Our results suggest that some of the proteins enriched in Br no. 19 may be involved in anthocyanin synthesis in the black glutinous rice.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006en10.1016/j.ejbt.2014.11.005
institution Scielo Chile
collection Scielo Chile
language English
topic 2D-PAGE
Anthocyanin
LC-MS/MS
Oryza sativa L
Proteomics
spellingShingle 2D-PAGE
Anthocyanin
LC-MS/MS
Oryza sativa L
Proteomics
Phonsakhan,Wipada
Kong-Ngern,Kanlaya
A comparative proteomic study of white and black glutinous rice leaves
description Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5 software. The results showed that the protein profiles of these two rice cultivars were different, with at least six protein spots that were detected only in Br no. 19. In addition, seven protein spots accumulated at higher levels in Br no. 19 than in RD 6. Conclusion The protein spot S1 (AP005098.4) is homologous to the Rc protein. Our results suggest that some of the proteins enriched in Br no. 19 may be involved in anthocyanin synthesis in the black glutinous rice.
author Phonsakhan,Wipada
Kong-Ngern,Kanlaya
author_facet Phonsakhan,Wipada
Kong-Ngern,Kanlaya
author_sort Phonsakhan,Wipada
title A comparative proteomic study of white and black glutinous rice leaves
title_short A comparative proteomic study of white and black glutinous rice leaves
title_full A comparative proteomic study of white and black glutinous rice leaves
title_fullStr A comparative proteomic study of white and black glutinous rice leaves
title_full_unstemmed A comparative proteomic study of white and black glutinous rice leaves
title_sort comparative proteomic study of white and black glutinous rice leaves
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006
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