Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning

Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) ana...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cheng,Jingliang, Long,Yan, Khan,Asaduzzaman, Wei,Chunli, Fu,Shelly, Fu,Junjiang
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Genetic authentication Molecular cloning Random amplified polymorphic DNA Sequence-characterized amplified region marker
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582015000100007
record_format	dspace
spelling	oai:scielo:S0717-345820150001000072015-03-09Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloningCheng,JingliangLong,YanKhan,AsaduzzamanWei,ChunliFu,ShellyFu,Junjiang Genetic authentication Molecular cloning Random amplified polymorphic DNA Sequence-characterized amplified region marker Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. The goal of this study was to develop sequence-characterized amplified region (SCAR) markers from the improved RAPD fragments for the genetic analysis of L. chinensis. Results The improved RAPD fragments from L. chinensis were cloned, sequenced and converted into stable SCAR markers. Sequencing of three cloned RAPD fragments revealed that the clone L7-16 consisted of 222 nucleotides (GenBank accession number KM235222), clone L9-6 consisted of 648 nucleotides (GenBank accession number KM235223), and clone L11-26 consisted of 369 nucleotides (GenBank accession number KM235224). Then, specific primers for SCAR markers L7-16, L9-6, and L11-26 were designed and synthesized. PCR amplification was performed using DNA templates from 24 different samples, including 6 samples of L. chinensis and other plants. The SCAR marker L9-6 was specific for all of the L. chinensis samples, the SCAR marker L11-26 specific for five L. chinensis samples, and the SCAR marker L7-16 only specific for the samples from Luzhou. Conclusions This study developed stable SCAR markers for the identification of L. chinensis by the cloning of the improved RAPD fragments. Combining RAPD and SCAR markers provides a simple and reliable tool for the genetic characterization of plant species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007en10.1016/j.ejbt.2014.11.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic authentication Molecular cloning Random amplified polymorphic DNA Sequence-characterized amplified region marker
spellingShingle	Genetic authentication Molecular cloning Random amplified polymorphic DNA Sequence-characterized amplified region marker Cheng,Jingliang Long,Yan Khan,Asaduzzaman Wei,Chunli Fu,Shelly Fu,Junjiang Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
description	Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. The goal of this study was to develop sequence-characterized amplified region (SCAR) markers from the improved RAPD fragments for the genetic analysis of L. chinensis. Results The improved RAPD fragments from L. chinensis were cloned, sequenced and converted into stable SCAR markers. Sequencing of three cloned RAPD fragments revealed that the clone L7-16 consisted of 222 nucleotides (GenBank accession number KM235222), clone L9-6 consisted of 648 nucleotides (GenBank accession number KM235223), and clone L11-26 consisted of 369 nucleotides (GenBank accession number KM235224). Then, specific primers for SCAR markers L7-16, L9-6, and L11-26 were designed and synthesized. PCR amplification was performed using DNA templates from 24 different samples, including 6 samples of L. chinensis and other plants. The SCAR marker L9-6 was specific for all of the L. chinensis samples, the SCAR marker L11-26 specific for five L. chinensis samples, and the SCAR marker L7-16 only specific for the samples from Luzhou. Conclusions This study developed stable SCAR markers for the identification of L. chinensis by the cloning of the improved RAPD fragments. Combining RAPD and SCAR markers provides a simple and reliable tool for the genetic characterization of plant species.
author	Cheng,Jingliang Long,Yan Khan,Asaduzzaman Wei,Chunli Fu,Shelly Fu,Junjiang
author_facet	Cheng,Jingliang Long,Yan Khan,Asaduzzaman Wei,Chunli Fu,Shelly Fu,Junjiang
author_sort	Cheng,Jingliang
title	Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
title_short	Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
title_full	Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
title_fullStr	Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
title_full_unstemmed	Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning
title_sort	development and significance of rapd-scar markers for the identification of litchi chinensis sonn: by improved rapd amplification and molecular cloning
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007
work_keys_str_mv	AT chengjingliang developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning AT longyan developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning AT khanasaduzzaman developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning AT weichunli developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning AT fushelly developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning AT fujunjiang developmentandsignificanceofrapdscarmarkersfortheidentificationoflitchichinensissonnbyimprovedrapdamplificationandmolecularcloning
_version_	1718441902207926272

Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning

Ejemplares similares