Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

Background Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines and F1 hybrids respectively. Cytoplasmic male sterility system (CMS) is one of the most important pol...

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Autores principales:	Havlíčková,Lenka, Klíma,Miroslav, Přibylová,Marie, Hilgert-Delgado,Alois Antonín, Kučera,Vratislav, Čurn,Vladislav
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Cytoplasmic male sterility Microspore embryogenesis Ogura CMS Oilseed rape Rfo gene
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100011
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spelling	oai:scielo:S0717-345820150001000112015-03-09Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)Havlíčková,LenkaKlíma,MiroslavPřibylová,MarieHilgert-Delgado,Alois AntonínKučera,VratislavČurn,Vladislav Cytoplasmic male sterility Microspore embryogenesis Ogura CMS Oilseed rape Rfo gene Background Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines and F1 hybrids respectively. Cytoplasmic male sterility system (CMS) is one of the most important pollination systems for hybrid seed production and utilisation of doubled haploid system to quickly prepare fully homozygous fertility restorer lines for CMS Ogu-INRA is very beneficial. Generally, only a small part of microspore-derived embryos is used for plant regeneration, without any knowledge about their properties. Therefore, the possibility of early detection of desirable genotypes bearing a single dominant nuclear fertility restorer (Rfo) gene, can double the success of selection and reduce the production costs. Results To maximize the efficiency and yield of regenerated microspore-derived embryos (MDEs) with the Rfo gene, a protocol for reliable and early, non-destructive selection of desired MDE genotypes was developed. The total amount of 636 cotyledonary embryos was tested by PCR, out of which 37% (237/636) were shown to bear the Rfo gene (instead of 50% according to the expected 1:1 segregation ratio for a single copy gene) and 218 of these fertility restorer plants were fully grown to flowering stage. New molecular marker has been demonstrated to have 100% of co-segregation with the phenotypic evaluation. Conclusion Technique developed in this study provides early and non-destructive sampling of embryonic tissue and the use of new markers for simple and efficient control of the presence of Rfo gene in all accessions.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.1 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100011en10.1016/j.ejbt.2014.10.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cytoplasmic male sterility Microspore embryogenesis Ogura CMS Oilseed rape Rfo gene
spellingShingle	Cytoplasmic male sterility Microspore embryogenesis Ogura CMS Oilseed rape Rfo gene Havlíčková,Lenka Klíma,Miroslav Přibylová,Marie Hilgert-Delgado,Alois Antonín Kučera,Vratislav Čurn,Vladislav Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
description	Background Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines and F1 hybrids respectively. Cytoplasmic male sterility system (CMS) is one of the most important pollination systems for hybrid seed production and utilisation of doubled haploid system to quickly prepare fully homozygous fertility restorer lines for CMS Ogu-INRA is very beneficial. Generally, only a small part of microspore-derived embryos is used for plant regeneration, without any knowledge about their properties. Therefore, the possibility of early detection of desirable genotypes bearing a single dominant nuclear fertility restorer (Rfo) gene, can double the success of selection and reduce the production costs. Results To maximize the efficiency and yield of regenerated microspore-derived embryos (MDEs) with the Rfo gene, a protocol for reliable and early, non-destructive selection of desired MDE genotypes was developed. The total amount of 636 cotyledonary embryos was tested by PCR, out of which 37% (237/636) were shown to bear the Rfo gene (instead of 50% according to the expected 1:1 segregation ratio for a single copy gene) and 218 of these fertility restorer plants were fully grown to flowering stage. New molecular marker has been demonstrated to have 100% of co-segregation with the phenotypic evaluation. Conclusion Technique developed in this study provides early and non-destructive sampling of embryonic tissue and the use of new markers for simple and efficient control of the presence of Rfo gene in all accessions.
author	Havlíčková,Lenka Klíma,Miroslav Přibylová,Marie Hilgert-Delgado,Alois Antonín Kučera,Vratislav Čurn,Vladislav
author_facet	Havlíčková,Lenka Klíma,Miroslav Přibylová,Marie Hilgert-Delgado,Alois Antonín Kučera,Vratislav Čurn,Vladislav
author_sort	Havlíčková,Lenka
title	Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
title_short	Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
title_full	Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
title_fullStr	Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
title_full_unstemmed	Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)
title_sort	non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for cms ogu-inra in winter oilseed rape (brassica napus l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100011
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Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

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