DNA from tissues of young mice is optimal for genotyping

Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We...

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Autores principales:	Picazo,María G, García-Olmo,Dolores C
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	DNA extraction Genotyping Mouse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004
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spelling	oai:scielo:S0717-345820150002000042015-05-08DNA from tissues of young mice is optimal for genotypingPicazo,María GGarcía-Olmo,Dolores C DNA extraction Genotyping Mouse Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues from YM yielded more DNA than those from AM, with differences being statistically significant for ear samples (38 ± 12 ng/μL for YM; 7 ± 3 ng/μL for AM; P = 0.006). In YM, the most DNA was obtained from ear and tail samples, with differences from the amounts obtained from phalanx samples being statistically significant (P = 0.02 and P = 0.005, respectively). In AM, the most DNA was obtained from tail and blood samples. Samples obtained by non-invasive sampling methods in adults resulted in a deficient DNA extraction. Conclusions The results of the present study do not support the previous recommendations for using non-invasive methods to genotype adult animals. The use of newborn tissue samples showed the highest efficiency for DNA extraction.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004en10.1016/j.ejbt.2014.12.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA extraction Genotyping Mouse
spellingShingle	DNA extraction Genotyping Mouse Picazo,María G García-Olmo,Dolores C DNA from tissues of young mice is optimal for genotyping
description	Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues from YM yielded more DNA than those from AM, with differences being statistically significant for ear samples (38 ± 12 ng/μL for YM; 7 ± 3 ng/μL for AM; P = 0.006). In YM, the most DNA was obtained from ear and tail samples, with differences from the amounts obtained from phalanx samples being statistically significant (P = 0.02 and P = 0.005, respectively). In AM, the most DNA was obtained from tail and blood samples. Samples obtained by non-invasive sampling methods in adults resulted in a deficient DNA extraction. Conclusions The results of the present study do not support the previous recommendations for using non-invasive methods to genotype adult animals. The use of newborn tissue samples showed the highest efficiency for DNA extraction.
author	Picazo,María G García-Olmo,Dolores C
author_facet	Picazo,María G García-Olmo,Dolores C
author_sort	Picazo,María G
title	DNA from tissues of young mice is optimal for genotyping
title_short	DNA from tissues of young mice is optimal for genotyping
title_full	DNA from tissues of young mice is optimal for genotyping
title_fullStr	DNA from tissues of young mice is optimal for genotyping
title_full_unstemmed	DNA from tissues of young mice is optimal for genotyping
title_sort	dna from tissues of young mice is optimal for genotyping
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004
work_keys_str_mv	AT picazomariag dnafromtissuesofyoungmiceisoptimalforgenotyping AT garciaolmodoloresc dnafromtissuesofyoungmiceisoptimalforgenotyping
_version_	1718441904532619264

DNA from tissues of young mice is optimal for genotyping

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