Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities

Background Triterpenoids are multifunctional secondary metabolites in plants. But little information is available concerning the actual yield, optimal extraction method and pharmacologic activity with regard to triterpenoids from Jatropha curcas leaves (TJL). Hence, response surface methodology (RSM...

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Autores principales:	Wei,Lei, Zhang,Wei, Yin,Li, Yan,Fang, Xu,Ying, Chen,Fang
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Box-Behnken design Radical scavenging activity Ultrasound-assistant extraction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200005
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spelling	oai:scielo:S0717-345820150002000052015-05-08Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacitiesWei,LeiZhang,WeiYin,LiYan,FangXu,YingChen,Fang Box-Behnken design Radical scavenging activity Ultrasound-assistant extraction Background Triterpenoids are multifunctional secondary metabolites in plants. But little information is available concerning the actual yield, optimal extraction method and pharmacologic activity with regard to triterpenoids from Jatropha curcas leaves (TJL). Hence, response surface methodology (RSM) was used to optimize the extraction parameters. The effects of three independent variables, namely liquid-to-solid ratio, ethanol concentration and extraction time on TJL yield were investigated. TJL obtained by silica column chromatography was tested against bacterial and fungal species relevant to oral disease and wounds through broth microdilution. Antioxidant activity was assessed using the 2,2-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Results A second order polynomial model produced a satisfactory fitting of the experimental data with regard to TJL yield (R2 = 0.983, P < 0.01). The optimum extraction conditions were 16 mL/g (liquid-to-solid ratio), 70% (ethanol concentration) and 50 min (extraction time). Predicted values agreed well with the experimental values. TJL had extraordinarily strong antibacterial and antifungal activities (24.42 µg/mL < MIC < 195.31 µg/mL) against all the tested human pathogens except Bacteroides vulgatus (390.62 µg/mL) and Bacteroides stercoris (781.25 µg/mL). The DPPH and ABTS assays revealed a moderate antioxidant activity of TJL compared with ascorbic acid. Conclusion These results provided reliable scientific basis for further investigation of triterpenoids from J. curcas.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200005en10.1016/j.ejbt.2014.12.005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Box-Behnken design Radical scavenging activity Ultrasound-assistant extraction
spellingShingle	Box-Behnken design Radical scavenging activity Ultrasound-assistant extraction Wei,Lei Zhang,Wei Yin,Li Yan,Fang Xu,Ying Chen,Fang Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
description	Background Triterpenoids are multifunctional secondary metabolites in plants. But little information is available concerning the actual yield, optimal extraction method and pharmacologic activity with regard to triterpenoids from Jatropha curcas leaves (TJL). Hence, response surface methodology (RSM) was used to optimize the extraction parameters. The effects of three independent variables, namely liquid-to-solid ratio, ethanol concentration and extraction time on TJL yield were investigated. TJL obtained by silica column chromatography was tested against bacterial and fungal species relevant to oral disease and wounds through broth microdilution. Antioxidant activity was assessed using the 2,2-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. Results A second order polynomial model produced a satisfactory fitting of the experimental data with regard to TJL yield (R2 = 0.983, P < 0.01). The optimum extraction conditions were 16 mL/g (liquid-to-solid ratio), 70% (ethanol concentration) and 50 min (extraction time). Predicted values agreed well with the experimental values. TJL had extraordinarily strong antibacterial and antifungal activities (24.42 µg/mL < MIC < 195.31 µg/mL) against all the tested human pathogens except Bacteroides vulgatus (390.62 µg/mL) and Bacteroides stercoris (781.25 µg/mL). The DPPH and ABTS assays revealed a moderate antioxidant activity of TJL compared with ascorbic acid. Conclusion These results provided reliable scientific basis for further investigation of triterpenoids from J. curcas.
author	Wei,Lei Zhang,Wei Yin,Li Yan,Fang Xu,Ying Chen,Fang
author_facet	Wei,Lei Zhang,Wei Yin,Li Yan,Fang Xu,Ying Chen,Fang
author_sort	Wei,Lei
title	Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
title_short	Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
title_full	Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
title_fullStr	Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
title_full_unstemmed	Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
title_sort	extraction optimization of total triterpenoids from jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200005
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Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities

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