Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility

Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as o...

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Autores principales:	Ladeira,Silvania A, Cruz,Erica, Delatorre,Andréia B, Barbosa,João B, Leal Martins,Meire Lelis
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Avicelase Carboxymethylcellulase Corn steep liquor Sugarcane bagasse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200008
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spelling	oai:scielo:S0717-345820150002000082015-05-08Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibilityLadeira,Silvania ACruz,EricaDelatorre,Andréia BBarbosa,João BLeal Martins,Meire Lelis Avicelase Carboxymethylcellulase Corn steep liquor Sugarcane bagasse Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as other suitable applications. Results Bacillus sp. produced cellulases when cultivated at 50°C in liquid cultures containing sugarcane bagasse and corn steep liquor. Maximum avicelase (0.83 U mL-1) and CMCase (0.29 U mL-1) activities were reached in 120 h and 168 h of culturing time, respectively. The avicelase and CMCase presented an optimum activity at pH of 7.5 and 8.0, respectively. The maximum stability of avicelase and CMCase was observed at a pH range between 6.5-8.0 and 7.0-9.0 respectively, where they retained more than 70% of their maximum activities after incubation at room temperature for 3 h. The optimum temperature of avicelase and CMCase was 70°C, and both enzymes remained 100% stable until the treatment at 60°C for 1 h. Bacillus sp. cultures also released proteases into the culture medium, but the cellulases were resistant to protease digestion. The compatibility of cellulases varied with each laundry detergent tested, being more stable in the presence of Ultra Biz® and less with Ariel®. In addition, the enzyme was stable in sodium dodecyl sulfate and RENEX-95, and was inhibited by TritonX-100 and H2O2. Conclusions The properties presented by Bacillus sp. SMIA-2 suggest that this organism might become a potential source of lignocellulose-degrading enzymes for industrial applications such as in the detergent industry.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200008en10.1016/j.ejbt.2014.12.008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Avicelase Carboxymethylcellulase Corn steep liquor Sugarcane bagasse
spellingShingle	Avicelase Carboxymethylcellulase Corn steep liquor Sugarcane bagasse Ladeira,Silvania A Cruz,Erica Delatorre,Andréia B Barbosa,João B Leal Martins,Meire Lelis Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
description	Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as other suitable applications. Results Bacillus sp. produced cellulases when cultivated at 50°C in liquid cultures containing sugarcane bagasse and corn steep liquor. Maximum avicelase (0.83 U mL-1) and CMCase (0.29 U mL-1) activities were reached in 120 h and 168 h of culturing time, respectively. The avicelase and CMCase presented an optimum activity at pH of 7.5 and 8.0, respectively. The maximum stability of avicelase and CMCase was observed at a pH range between 6.5-8.0 and 7.0-9.0 respectively, where they retained more than 70% of their maximum activities after incubation at room temperature for 3 h. The optimum temperature of avicelase and CMCase was 70°C, and both enzymes remained 100% stable until the treatment at 60°C for 1 h. Bacillus sp. cultures also released proteases into the culture medium, but the cellulases were resistant to protease digestion. The compatibility of cellulases varied with each laundry detergent tested, being more stable in the presence of Ultra Biz® and less with Ariel®. In addition, the enzyme was stable in sodium dodecyl sulfate and RENEX-95, and was inhibited by TritonX-100 and H2O2. Conclusions The properties presented by Bacillus sp. SMIA-2 suggest that this organism might become a potential source of lignocellulose-degrading enzymes for industrial applications such as in the detergent industry.
author	Ladeira,Silvania A Cruz,Erica Delatorre,Andréia B Barbosa,João B Leal Martins,Meire Lelis
author_facet	Ladeira,Silvania A Cruz,Erica Delatorre,Andréia B Barbosa,João B Leal Martins,Meire Lelis
author_sort	Ladeira,Silvania A
title	Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
title_short	Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
title_full	Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
title_fullStr	Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
title_full_unstemmed	Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
title_sort	cellulase production by thermophilic bacillus sp: smia-2 and its detergent compatibility
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200008
work_keys_str_mv	AT ladeirasilvaniaa cellulaseproductionbythermophilicbacillusspsmia2anditsdetergentcompatibility AT cruzerica cellulaseproductionbythermophilicbacillusspsmia2anditsdetergentcompatibility AT delatorreandreiab cellulaseproductionbythermophilicbacillusspsmia2anditsdetergentcompatibility AT barbosajoaob cellulaseproductionbythermophilicbacillusspsmia2anditsdetergentcompatibility AT lealmartinsmeirelelis cellulaseproductionbythermophilicbacillusspsmia2anditsdetergentcompatibility
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Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility

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