The power of 28 microsatellite markers for parentage testing in sheep

Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common proble...

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Autor principal:	Al-Atiyat,Raed M
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Merino sheep Paternity verification Pedigree records SSR markers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200009
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spelling	oai:scielo:S0717-345820150002000092015-05-08The power of 28 microsatellite markers for parentage testing in sheepAl-Atiyat,Raed M Merino sheep Paternity verification Pedigree records SSR markers Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common problem of significant error in pedigree records in Merino sheep. Three different flocks of Australian Merino sheep were investigated. A private farm flock represents a flock with no record availability. Two other flocks were maintained under good managements of full keeping records and being selected for high and low parasite resistances. Results In the studied panel, eight MSTs provided an average of Polymorphic Information content (PIC) equal to 0.65 or more in order to be sufficient to make an accurate and successful DNA-based parentage analysis. The panel of twenty-eight MST loci was obviously sufficient for providing 100% accurate pedigree and genotyping data. DNA-based pedigree records were constructed and all significant pedigree record errors were eliminated. Conclusions These results were used for further study of population genetic parameters such as recombination and haplotyping which heavily based on pedigree information. Nevertheless MST based parentage testing is still available and affordable in most countries and for each farmer with reasonable cost in comparison with fast growing SNP based parentage technologies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200009en10.1016/j.ejbt.2015.01.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Merino sheep Paternity verification Pedigree records SSR markers
spellingShingle	Merino sheep Paternity verification Pedigree records SSR markers Al-Atiyat,Raed M The power of 28 microsatellite markers for parentage testing in sheep
description	Background In sheep breeding, there are situations where relationships recorded at the farm among pedigrees such as parent-offspring, full-sibs or half-sibs need to be tested. A panel of 28 microsatellite (MST) markers was tested to provide accurate pedigree information and resolve the common problem of significant error in pedigree records in Merino sheep. Three different flocks of Australian Merino sheep were investigated. A private farm flock represents a flock with no record availability. Two other flocks were maintained under good managements of full keeping records and being selected for high and low parasite resistances. Results In the studied panel, eight MSTs provided an average of Polymorphic Information content (PIC) equal to 0.65 or more in order to be sufficient to make an accurate and successful DNA-based parentage analysis. The panel of twenty-eight MST loci was obviously sufficient for providing 100% accurate pedigree and genotyping data. DNA-based pedigree records were constructed and all significant pedigree record errors were eliminated. Conclusions These results were used for further study of population genetic parameters such as recombination and haplotyping which heavily based on pedigree information. Nevertheless MST based parentage testing is still available and affordable in most countries and for each farmer with reasonable cost in comparison with fast growing SNP based parentage technologies.
author	Al-Atiyat,Raed M
author_facet	Al-Atiyat,Raed M
author_sort	Al-Atiyat,Raed M
title	The power of 28 microsatellite markers for parentage testing in sheep
title_short	The power of 28 microsatellite markers for parentage testing in sheep
title_full	The power of 28 microsatellite markers for parentage testing in sheep
title_fullStr	The power of 28 microsatellite markers for parentage testing in sheep
title_full_unstemmed	The power of 28 microsatellite markers for parentage testing in sheep
title_sort	power of 28 microsatellite markers for parentage testing in sheep
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200009
work_keys_str_mv	AT alatiyatraedm thepowerof28microsatellitemarkersforparentagetestinginsheep AT alatiyatraedm powerof28microsatellitemarkersforparentagetestinginsheep
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The power of 28 microsatellite markers for parentage testing in sheep

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