PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development

Background The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Xu,Xiaochun, Wei,Xuan, Yang,Yuxin, Niu,Wenzhi, Kou,Qifang, Wang,Xiaolong, Chen,Yulin
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2015
Materias:
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200010
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0717-34582015000200010
record_format dspace
spelling oai:scielo:S0717-345820150002000102015-05-08PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail developmentXu,XiaochunWei,XuanYang,YuxinNiu,WenzhiKou,QifangWang,XiaolongChen,Yulin Fat-tailed sheep mRNA transcription Protein expression Transcription factor and enzyme genes Background The objective of this study was to investigate proliferator-activated receptor (PPAR&#947;), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPAR&#947;, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results The tail measurements increased with age. We observed no significant differences (P &gt; 0.05) of PPAR&#947; mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS mRNA expression levels at each developmental stage were observed significantly in Tan sheep (P < 0.05); HSL mRNA expression with no significant differences were only observed between 6 and 15 months (P &gt; 0.05). Significant differences (P < 0.05) of PPAR&#947;, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep. Conclusion We observed that the mRNA expression patterns of PPAR&#947; and FAS decreased first before they increased again and then this process repeated. Conversely, the mRNA expression patterns of HSL increased first before they decreased and then this process repeated. The protein expression patterns of PPAR&#947; and FAS decreased first before they increased again and then this process repeated. Conversely, the protein expression pattern of HSL increased first before it decreased again and then increased again.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200010en10.1016/j.ejbt.2015.01.004
institution Scielo Chile
collection Scielo Chile
language English
topic Fat-tailed sheep
mRNA transcription
Protein expression
Transcription factor and enzyme genes
spellingShingle Fat-tailed sheep
mRNA transcription
Protein expression
Transcription factor and enzyme genes
Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
description Background The objective of this study was to investigate proliferator-activated receptor (PPAR&#947;), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPAR&#947;, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results The tail measurements increased with age. We observed no significant differences (P &gt; 0.05) of PPAR&#947; mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS mRNA expression levels at each developmental stage were observed significantly in Tan sheep (P < 0.05); HSL mRNA expression with no significant differences were only observed between 6 and 15 months (P &gt; 0.05). Significant differences (P < 0.05) of PPAR&#947;, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep. Conclusion We observed that the mRNA expression patterns of PPAR&#947; and FAS decreased first before they increased again and then this process repeated. Conversely, the mRNA expression patterns of HSL increased first before they decreased and then this process repeated. The protein expression patterns of PPAR&#947; and FAS decreased first before they increased again and then this process repeated. Conversely, the protein expression pattern of HSL increased first before it decreased again and then increased again.
author Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
author_facet Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
author_sort Xu,Xiaochun
title PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
title_short PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
title_full PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
title_fullStr PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
title_full_unstemmed PPAR&#947;, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development
title_sort ppar&#947;, fas, hsl mrna and protein expression during tan sheep fat-tail development
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200010
work_keys_str_mv AT xuxiaochun ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT weixuan ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT yangyuxin ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT niuwenzhi ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT kouqifang ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT wangxiaolong ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
AT chenyulin ppar947fashslmrnaandproteinexpressionduringtansheepfattaildevelopment
_version_ 1718441906068783104