Total RNA quality of lyophilized and cryopreserved dormant grapevine buds

Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the tota...

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Autores principales:	García-Baldenegro,Claudia Vanessa, Vargas-Arispuro,Irasema, Islas-Osuna,María, Rivera-Domínguez,Marisela, Aispuro-Hernández,Emmanuel, Martínez-Téllez,Miguel Ángel
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Freeze-dried RNA integrity number RT-qPCR Vitis vinifera L Woody tissue
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012
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id	oai:scielo:S0717-34582015000200012
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spelling	oai:scielo:S0717-345820150002000122015-05-08Total RNA quality of lyophilized and cryopreserved dormant grapevine budsGarcía-Baldenegro,Claudia VanessaVargas-Arispuro,IrasemaIslas-Osuna,MaríaRivera-Domínguez,MariselaAispuro-Hernández,EmmanuelMartínez-Téllez,Miguel Ángel Freeze-dried RNA integrity number RT-qPCR Vitis vinifera L Woody tissue Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds (Vitis vinifera L. cv. Flame Seedless) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds. Results Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and β-tubulin PCR gene amplification. Conclusion High-quality RNA that is useful for downstream applications was obtained from freeze-dried dormant grapevine bud tissue, similarly to the RNA obtained from cryopreserved dormant grapevine buds.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.2 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012en10.1016/j.ejbt.2015.01.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Freeze-dried RNA integrity number RT-qPCR Vitis vinifera L Woody tissue
spellingShingle	Freeze-dried RNA integrity number RT-qPCR Vitis vinifera L Woody tissue García-Baldenegro,Claudia Vanessa Vargas-Arispuro,Irasema Islas-Osuna,María Rivera-Domínguez,Marisela Aispuro-Hernández,Emmanuel Martínez-Téllez,Miguel Ángel Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
description	Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds (Vitis vinifera L. cv. Flame Seedless) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds. Results Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and β-tubulin PCR gene amplification. Conclusion High-quality RNA that is useful for downstream applications was obtained from freeze-dried dormant grapevine bud tissue, similarly to the RNA obtained from cryopreserved dormant grapevine buds.
author	García-Baldenegro,Claudia Vanessa Vargas-Arispuro,Irasema Islas-Osuna,María Rivera-Domínguez,Marisela Aispuro-Hernández,Emmanuel Martínez-Téllez,Miguel Ángel
author_facet	García-Baldenegro,Claudia Vanessa Vargas-Arispuro,Irasema Islas-Osuna,María Rivera-Domínguez,Marisela Aispuro-Hernández,Emmanuel Martínez-Téllez,Miguel Ángel
author_sort	García-Baldenegro,Claudia Vanessa
title	Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
title_short	Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
title_full	Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
title_fullStr	Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
title_full_unstemmed	Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
title_sort	total rna quality of lyophilized and cryopreserved dormant grapevine buds
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012
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Total RNA quality of lyophilized and cryopreserved dormant grapevine buds

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