Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks

Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao,Anfeng, Huang,Yufeng, Ni,Hui, Cai,Huinong, Yang,Qiuming
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582015000300001
record_format	dspace
spelling	oai:scielo:S0717-345820150003000012015-06-26Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalksXiao,AnfengHuang,YufengNi,HuiCai,HuinongYang,Qiuming Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R²) of 0.9340, which implied an adequate credibility of the model.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001en10.1016/j.ejbt.2015.02.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation
spellingShingle	Aspergillus tubingensis Process optimization Response surface methodology Tea stalks Tannase fermentation Xiao,Anfeng Huang,Yufeng Ni,Hui Cai,Huinong Yang,Qiuming Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
description	Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds). The results were fitted onto a second-order polynomial model with a correlation coefficient (R²) of 0.9340, which implied an adequate credibility of the model.
author	Xiao,Anfeng Huang,Yufeng Ni,Hui Cai,Huinong Yang,Qiuming
author_facet	Xiao,Anfeng Huang,Yufeng Ni,Hui Cai,Huinong Yang,Qiuming
author_sort	Xiao,Anfeng
title	Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
title_short	Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
title_full	Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
title_fullStr	Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
title_full_unstemmed	Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks
title_sort	statistical optimization for tannase production by aspergillus tubingensis in solid-state fermentation using tea stalks
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001
work_keys_str_mv	AT xiaoanfeng statisticaloptimizationfortannaseproductionbyaspergillustubingensisinsolidstatefermentationusingteastalks AT huangyufeng statisticaloptimizationfortannaseproductionbyaspergillustubingensisinsolidstatefermentationusingteastalks AT nihui statisticaloptimizationfortannaseproductionbyaspergillustubingensisinsolidstatefermentationusingteastalks AT caihuinong statisticaloptimizationfortannaseproductionbyaspergillustubingensisinsolidstatefermentationusingteastalks AT yangqiuming statisticaloptimizationfortannaseproductionbyaspergillustubingensisinsolidstatefermentationusingteastalks
_version_	1718441907013550080

Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks

Ejemplares similares