Overproduction of clavulanic acid by extractive fermentation

Background Clavulanic acid is an important beta-lactamase inhibitor produced as a secondary metabolite by the actinomycete Streptomyces clavuligerus. Clavulanic acid is chemically unstable; therefore, it is degraded during bacterial cultivation. In this work, the adsorbents clinoptilolite, activated...

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Autores principales:	Lopes Costa,Cecília Ladeira, Colli Badino,Alberto
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Adsorption Clavulanic acid Extractive fermentation Integrated processing Product inhibition
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300003
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spelling	oai:scielo:S0717-345820150003000032015-06-26Overproduction of clavulanic acid by extractive fermentationLopes Costa,Cecília LadeiraColli Badino,Alberto Adsorption Clavulanic acid Extractive fermentation Integrated processing Product inhibition Background Clavulanic acid is an important beta-lactamase inhibitor produced as a secondary metabolite by the actinomycete Streptomyces clavuligerus. Clavulanic acid is chemically unstable; therefore, it is degraded during bacterial cultivation. In this work, the adsorbents clinoptilolite, activated carbon, calcined hydrotalcite, and Amberlite IRA 400 anionic exchange resin were studied in terms of their ability to adsorb clavulanic acid during extractive fermentation, in order to prevent product degradation and avoid product concentrations reaching inhibitory levels. Adsorption assays were used to investigate the effect of pH, and the decrease in the clavulanic acid concentration in the culture broth was measured for each adsorbent. Results IRA 400 was found to be most effective, with 78% adsorption of clavulanic acid. The maximum production of clavulanic acid in Erlenmeyer flask cultures increased 86% in terms of mass of CA, and 248% in cumulative CA concentration, with the use of Amberlite IRA 400 as adsorbent in extractive fermentation, compared to control fermentation performed without product removal. Conclusions The results indicated that extractive fermentation using a solid phase could be an important way of enhancing clavulanic acid titers. It was also possible to show that clavulanic acid acts as an inhibitor of its own synthesis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300003en10.1016/j.ejbt.2015.03.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adsorption Clavulanic acid Extractive fermentation Integrated processing Product inhibition
spellingShingle	Adsorption Clavulanic acid Extractive fermentation Integrated processing Product inhibition Lopes Costa,Cecília Ladeira Colli Badino,Alberto Overproduction of clavulanic acid by extractive fermentation
description	Background Clavulanic acid is an important beta-lactamase inhibitor produced as a secondary metabolite by the actinomycete Streptomyces clavuligerus. Clavulanic acid is chemically unstable; therefore, it is degraded during bacterial cultivation. In this work, the adsorbents clinoptilolite, activated carbon, calcined hydrotalcite, and Amberlite IRA 400 anionic exchange resin were studied in terms of their ability to adsorb clavulanic acid during extractive fermentation, in order to prevent product degradation and avoid product concentrations reaching inhibitory levels. Adsorption assays were used to investigate the effect of pH, and the decrease in the clavulanic acid concentration in the culture broth was measured for each adsorbent. Results IRA 400 was found to be most effective, with 78% adsorption of clavulanic acid. The maximum production of clavulanic acid in Erlenmeyer flask cultures increased 86% in terms of mass of CA, and 248% in cumulative CA concentration, with the use of Amberlite IRA 400 as adsorbent in extractive fermentation, compared to control fermentation performed without product removal. Conclusions The results indicated that extractive fermentation using a solid phase could be an important way of enhancing clavulanic acid titers. It was also possible to show that clavulanic acid acts as an inhibitor of its own synthesis.
author	Lopes Costa,Cecília Ladeira Colli Badino,Alberto
author_facet	Lopes Costa,Cecília Ladeira Colli Badino,Alberto
author_sort	Lopes Costa,Cecília Ladeira
title	Overproduction of clavulanic acid by extractive fermentation
title_short	Overproduction of clavulanic acid by extractive fermentation
title_full	Overproduction of clavulanic acid by extractive fermentation
title_fullStr	Overproduction of clavulanic acid by extractive fermentation
title_full_unstemmed	Overproduction of clavulanic acid by extractive fermentation
title_sort	overproduction of clavulanic acid by extractive fermentation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300003
work_keys_str_mv	AT lopescostacecilialadeira overproductionofclavulanicacidbyextractivefermentation AT collibadinoalberto overproductionofclavulanicacidbyextractivefermentation
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Overproduction of clavulanic acid by extractive fermentation

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