Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections

Background Enterococcus faecalis is considered to be one of most prevalent species in the oral cavity, particularly in endodontic infections. The aim of the present study was to investigate the prevalence of E. faecalis in dental root canals, clonal diversity by restriction fragment length polymorph...

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Autores principales:	Suliman Al-Badah,Abdulhakim, S.S. Ibrahim,Abdelnasser, Al-Salamah,Ali A, Ibrahim,Shebl Salah S
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Antimicrobial susceptibility Endodontic infection Enterococcus faecalis Genotype RAPD RFLP
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300006
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spelling	oai:scielo:S0717-345820150003000062015-06-26Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infectionsSuliman Al-Badah,AbdulhakimS.S. Ibrahim,AbdelnasserAl-Salamah,Ali AIbrahim,Shebl Salah S Antimicrobial susceptibility Endodontic infection Enterococcus faecalis Genotype RAPD RFLP Background Enterococcus faecalis is considered to be one of most prevalent species in the oral cavity, particularly in endodontic infections. The aim of the present study was to investigate the prevalence of E. faecalis in dental root canals, clonal diversity by restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD-PCR) analysis, and the antibiotic susceptibility of E. faecalis isolates. Results Among the bacterial strains isolated from dental root canal specimens (n = 82), E. faecalis was determined to have the highest prevalence followed by Streptococcus viridians, Leuconostoc mesenteroides, Staphylococcus aureus, Streptococcus mitis, and Pediococcus pentosaceus. Cluster analysis of RAPD-PCR and RFLP patterns of the E. faecalis isolates discriminated five and six different genotypes, respectively. Among the tested strains, 43%, 52% and 5% were susceptible, intermediate resistant, and resistant to erythromycin, respectively. In addition, one strain (E-12) was intermediate resistant to linezolid, and one isolate (E-16) was resistant to tetracycline. Interestingly, many of the intermediate resistant/resistant strains were grouped in clusters 5 and 6, according RAPD and to RFLP, respectively. Conclusions E. faecalis demonstrated the highest prevalence in the tested dental root canal specimens collected from Saudi patients and were grouped into five to six different genotypes. Different levels of antimicrobial susceptibility were observed in the tested E. faecalis strains, which clearly indicated that although bacterial strains may be similar, point mutations can result in extreme susceptibility or resistance to various antibiotics. This phenomenon is a cause for concern for clinicians in the treatment of dental infections caused by E. faecalis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300006en10.1016/j.ejbt.2015.03.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antimicrobial susceptibility Endodontic infection Enterococcus faecalis Genotype RAPD RFLP
spellingShingle	Antimicrobial susceptibility Endodontic infection Enterococcus faecalis Genotype RAPD RFLP Suliman Al-Badah,Abdulhakim S.S. Ibrahim,Abdelnasser Al-Salamah,Ali A Ibrahim,Shebl Salah S Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
description	Background Enterococcus faecalis is considered to be one of most prevalent species in the oral cavity, particularly in endodontic infections. The aim of the present study was to investigate the prevalence of E. faecalis in dental root canals, clonal diversity by restriction fragment length polymorphism (RFLP) and randomly amplified polymorphic DNA (RAPD-PCR) analysis, and the antibiotic susceptibility of E. faecalis isolates. Results Among the bacterial strains isolated from dental root canal specimens (n = 82), E. faecalis was determined to have the highest prevalence followed by Streptococcus viridians, Leuconostoc mesenteroides, Staphylococcus aureus, Streptococcus mitis, and Pediococcus pentosaceus. Cluster analysis of RAPD-PCR and RFLP patterns of the E. faecalis isolates discriminated five and six different genotypes, respectively. Among the tested strains, 43%, 52% and 5% were susceptible, intermediate resistant, and resistant to erythromycin, respectively. In addition, one strain (E-12) was intermediate resistant to linezolid, and one isolate (E-16) was resistant to tetracycline. Interestingly, many of the intermediate resistant/resistant strains were grouped in clusters 5 and 6, according RAPD and to RFLP, respectively. Conclusions E. faecalis demonstrated the highest prevalence in the tested dental root canal specimens collected from Saudi patients and were grouped into five to six different genotypes. Different levels of antimicrobial susceptibility were observed in the tested E. faecalis strains, which clearly indicated that although bacterial strains may be similar, point mutations can result in extreme susceptibility or resistance to various antibiotics. This phenomenon is a cause for concern for clinicians in the treatment of dental infections caused by E. faecalis.
author	Suliman Al-Badah,Abdulhakim S.S. Ibrahim,Abdelnasser Al-Salamah,Ali A Ibrahim,Shebl Salah S
author_facet	Suliman Al-Badah,Abdulhakim S.S. Ibrahim,Abdelnasser Al-Salamah,Ali A Ibrahim,Shebl Salah S
author_sort	Suliman Al-Badah,Abdulhakim
title	Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
title_short	Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
title_full	Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
title_fullStr	Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
title_full_unstemmed	Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections
title_sort	clonal diversity and antimicrobial resistance of enterococcus faecalis isolated from endodontic infections
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300006
work_keys_str_mv	AT sulimanalbadahabdulhakim clonaldiversityandantimicrobialresistanceofenterococcusfaecalisisolatedfromendodonticinfections AT ssibrahimabdelnasser clonaldiversityandantimicrobialresistanceofenterococcusfaecalisisolatedfromendodonticinfections AT alsalamahalia clonaldiversityandantimicrobialresistanceofenterococcusfaecalisisolatedfromendodonticinfections AT ibrahimsheblsalahs clonaldiversityandantimicrobialresistanceofenterococcusfaecalisisolatedfromendodonticinfections
_version_	1718441908319027200

Clonal diversity and antimicrobial resistance of Enterococcus faecalis isolated from endodontic infections

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