Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers

Background The genetic diversity and structure of 31 popcorn accessions of the germplasm bank of the State University of Maringá were assessed using 30 microsatellite primers. Results 127 alleles were identified from 30 evaluated loci. The number of alleles per locus ranged from two to eight. The ov...

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Autores principales:	da Silva,Tereza Aparecida, Belizário Cantagalli,Liriana, Saavedra,Javier, Lopes,Ana Daniela, Aparecida Mangolin,Claudete, Pires da Silva Machado,Maria de Fatima, Scapim,Carlos Alberto
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Genetic variability Maize Molecular marker
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300007
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spelling	oai:scielo:S0717-345820150003000072015-06-26Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markersda Silva,Tereza AparecidaBelizário Cantagalli,LirianaSaavedra,JavierLopes,Ana DanielaAparecida Mangolin,ClaudetePires da Silva Machado,Maria de FatimaScapim,Carlos Alberto Genetic variability Maize Molecular marker Background The genetic diversity and structure of 31 popcorn accessions of the germplasm bank of the State University of Maringá were assessed using 30 microsatellite primers. Results 127 alleles were identified from 30 evaluated loci. The number of alleles per locus ranged from two to eight. The overall mean of the polymorphic loci averaged 79.89%. The primers UMC1549 and UMC1072 detected polymorphism in all accessions analyzed. The mean observed heterozygosity ranged from 0.07 to 0.30 and the highest proportion of heterozygous plants was observed in accession BOZM 260 (Ho = 0.30). The analysis of molecular variance revealed that 60% of the total genetic variation was found within accessions and 40% was found between accessions. The Bayesian clustering approach grouped the 31 accessions into two genetically differentiated clusters. The dendrogram revealed that accessions TATU 2 and ARZM 05 083 are genetically less similar than the others. Conclusions The analysis allowed to identify microsatellite loci with high levels of heterozygosity (UMC1549 and UMC1072). These loci can be indicated as promising for detecting polymorphisms in popcorn accessions and in the monitoring of genetic improvement programs. Moreover, allowed to identify heterozygous accessions (BOZM 260), this accession showed allelic variation at all analyzed microsatellite loci and can be recommended for crosses with plants that have desirable agronomic characteristics, with a view to the broadening of the genetic base of popcorn accessions and developing new cultivars.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300007en10.1016/j.ejbt.2015.03.005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic variability Maize Molecular marker
spellingShingle	Genetic variability Maize Molecular marker da Silva,Tereza Aparecida Belizário Cantagalli,Liriana Saavedra,Javier Lopes,Ana Daniela Aparecida Mangolin,Claudete Pires da Silva Machado,Maria de Fatima Scapim,Carlos Alberto Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
description	Background The genetic diversity and structure of 31 popcorn accessions of the germplasm bank of the State University of Maringá were assessed using 30 microsatellite primers. Results 127 alleles were identified from 30 evaluated loci. The number of alleles per locus ranged from two to eight. The overall mean of the polymorphic loci averaged 79.89%. The primers UMC1549 and UMC1072 detected polymorphism in all accessions analyzed. The mean observed heterozygosity ranged from 0.07 to 0.30 and the highest proportion of heterozygous plants was observed in accession BOZM 260 (Ho = 0.30). The analysis of molecular variance revealed that 60% of the total genetic variation was found within accessions and 40% was found between accessions. The Bayesian clustering approach grouped the 31 accessions into two genetically differentiated clusters. The dendrogram revealed that accessions TATU 2 and ARZM 05 083 are genetically less similar than the others. Conclusions The analysis allowed to identify microsatellite loci with high levels of heterozygosity (UMC1549 and UMC1072). These loci can be indicated as promising for detecting polymorphisms in popcorn accessions and in the monitoring of genetic improvement programs. Moreover, allowed to identify heterozygous accessions (BOZM 260), this accession showed allelic variation at all analyzed microsatellite loci and can be recommended for crosses with plants that have desirable agronomic characteristics, with a view to the broadening of the genetic base of popcorn accessions and developing new cultivars.
author	da Silva,Tereza Aparecida Belizário Cantagalli,Liriana Saavedra,Javier Lopes,Ana Daniela Aparecida Mangolin,Claudete Pires da Silva Machado,Maria de Fatima Scapim,Carlos Alberto
author_facet	da Silva,Tereza Aparecida Belizário Cantagalli,Liriana Saavedra,Javier Lopes,Ana Daniela Aparecida Mangolin,Claudete Pires da Silva Machado,Maria de Fatima Scapim,Carlos Alberto
author_sort	da Silva,Tereza Aparecida
title	Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
title_short	Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
title_full	Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
title_fullStr	Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
title_full_unstemmed	Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
title_sort	population structure and genetic diversity of brazilian popcorn germplasm inferred by microsatellite markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300007
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Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers

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