Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties

Background The role of polysaccharides isolated from the Ganoderma species of fungi in innate immunity has recently become a topic of research. Although some work has been conducted concerning Ganoderma lucidum, the characteristics of polysaccharides isolated from Ganoderma neojaponicum (Imazeki) as...

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Autores principales:	Nik Ubaidillah,Nik Hafizah, Abdullah,Noorlidah, Sabaratnam,Vikineswary
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	β-Glucan Acute oral toxicity Macrophage cell line Phagocytosis Stirred tank reactor Total carbohydrate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300008
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spelling	oai:scielo:S0717-345820150003000082015-06-26Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory propertiesNik Ubaidillah,Nik HafizahAbdullah,NoorlidahSabaratnam,Vikineswary β-Glucan Acute oral toxicity Macrophage cell line Phagocytosis Stirred tank reactor Total carbohydrate Background The role of polysaccharides isolated from the Ganoderma species of fungi in innate immunity has recently become a topic of research. Although some work has been conducted concerning Ganoderma lucidum, the characteristics of polysaccharides isolated from Ganoderma neojaponicum (Imazeki) as immunomodulatory agents are largely unknown. The aims for this study were to isolate and characterize the intracellular polysaccharides (IPSs) and extracellular polysaccharides (EPSs) of G. neojaponicum from STR reactor. Results The production of EPS and IPS was optimized on day 4 of the cultivation time in 2 L STR reactor based on the amount of biomass yield, total carbohydrate, β-glucan and a-glucan content. Further analysis, both the EPSs and IPSs showed the enhancement on proliferation and increment of phagocytosis activities of macrophage (RAW264.7) cell lines. Using an oral toxicity test, we also observed that 2000 mg/kg body weight/day dosage of dried G. neojaponicum mycelium does not cause any significant toxic effects on Sprague-Dawley rats in 14 d of administration. Conclusion The findings of this study indicate that the IPSs and EPSs of G. neojaponicum have the potential to be used as immunomodulating agents to stimulate the innate immune system for fighting infectious diseases. The polysaccharides from G. neojaponicum have to be further commercially explored as an alternative for medicinal Ganoderma variety of G. lucidum production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300008en10.1016/j.ejbt.2015.03.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	β-Glucan Acute oral toxicity Macrophage cell line Phagocytosis Stirred tank reactor Total carbohydrate
spellingShingle	β-Glucan Acute oral toxicity Macrophage cell line Phagocytosis Stirred tank reactor Total carbohydrate Nik Ubaidillah,Nik Hafizah Abdullah,Noorlidah Sabaratnam,Vikineswary Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
description	Background The role of polysaccharides isolated from the Ganoderma species of fungi in innate immunity has recently become a topic of research. Although some work has been conducted concerning Ganoderma lucidum, the characteristics of polysaccharides isolated from Ganoderma neojaponicum (Imazeki) as immunomodulatory agents are largely unknown. The aims for this study were to isolate and characterize the intracellular polysaccharides (IPSs) and extracellular polysaccharides (EPSs) of G. neojaponicum from STR reactor. Results The production of EPS and IPS was optimized on day 4 of the cultivation time in 2 L STR reactor based on the amount of biomass yield, total carbohydrate, β-glucan and a-glucan content. Further analysis, both the EPSs and IPSs showed the enhancement on proliferation and increment of phagocytosis activities of macrophage (RAW264.7) cell lines. Using an oral toxicity test, we also observed that 2000 mg/kg body weight/day dosage of dried G. neojaponicum mycelium does not cause any significant toxic effects on Sprague-Dawley rats in 14 d of administration. Conclusion The findings of this study indicate that the IPSs and EPSs of G. neojaponicum have the potential to be used as immunomodulating agents to stimulate the innate immune system for fighting infectious diseases. The polysaccharides from G. neojaponicum have to be further commercially explored as an alternative for medicinal Ganoderma variety of G. lucidum production.
author	Nik Ubaidillah,Nik Hafizah Abdullah,Noorlidah Sabaratnam,Vikineswary
author_facet	Nik Ubaidillah,Nik Hafizah Abdullah,Noorlidah Sabaratnam,Vikineswary
author_sort	Nik Ubaidillah,Nik Hafizah
title	Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
title_short	Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
title_full	Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
title_fullStr	Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
title_full_unstemmed	Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties
title_sort	isolation of the intracellular and extracellular polysaccharides of ganoderma neojaponicum (imazeki) and characterization of their immunomodulatory properties
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300008
work_keys_str_mv	AT nikubaidillahnikhafizah isolationoftheintracellularandextracellularpolysaccharidesofganodermaneojaponicumimazekiandcharacterizationoftheirimmunomodulatoryproperties AT abdullahnoorlidah isolationoftheintracellularandextracellularpolysaccharidesofganodermaneojaponicumimazekiandcharacterizationoftheirimmunomodulatoryproperties AT sabaratnamvikineswary isolationoftheintracellularandextracellularpolysaccharidesofganodermaneojaponicumimazekiandcharacterizationoftheirimmunomodulatoryproperties
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Isolation of the intracellular and extracellular polysaccharides of Ganoderma neojaponicum (Imazeki) and characterization of their immunomodulatory properties

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