Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae

Background Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results In this study the influence of high temperature on the cellular and mit...

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Autores principales:	Zhang,Min, Shi,Jun, Jiang,Li
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Bioethanol Random amplified polymorphic DNA (RAPD) Yeast
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300010
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spelling	oai:scielo:S0717-345820150003000102015-06-26Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiaeZhang,MinShi,JunJiang,Li Bioethanol Random amplified polymorphic DNA (RAPD) Yeast Background Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains had a clear correlation with the yeast's intracellular trehalose content of the cell. Finally, random amplified polymorphic DNA (RAPD) was used to explore the genome differences between the YZ1 and YF31 strains. Conclusions Thus, the stability of the mitochondrial membrane and subsequently, the clearance ROS ability could be important factors for the viability of S. cerevisiae at high temperatures.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300010en10.1016/j.ejbt.2015.03.008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bioethanol Random amplified polymorphic DNA (RAPD) Yeast
spellingShingle	Bioethanol Random amplified polymorphic DNA (RAPD) Yeast Zhang,Min Shi,Jun Jiang,Li Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
description	Background Yeast strains are exposed to numerous environmental stresses during industrial alcoholic fermentation. High temperature accumulated acetic acid, enhanced the growth inhibition and decreased ethanol production. Results In this study the influence of high temperature on the cellular and mitochondrial membrane integrity of Saccharomyces cerevisiae as well as reactive oxygen species (ROS) formation was investigated to understand the mechanisms of the high temperature fermentation process. However, increasing the temperature to 42°C resulted in a clear decrease in the cytoplasmic and mitochondrial membrane potential and an increase in intracellular ROS formation. It was also determined that the different thermostability between YZ1 and YF31 strains had a clear correlation with the yeast's intracellular trehalose content of the cell. Finally, random amplified polymorphic DNA (RAPD) was used to explore the genome differences between the YZ1 and YF31 strains. Conclusions Thus, the stability of the mitochondrial membrane and subsequently, the clearance ROS ability could be important factors for the viability of S. cerevisiae at high temperatures.
author	Zhang,Min Shi,Jun Jiang,Li
author_facet	Zhang,Min Shi,Jun Jiang,Li
author_sort	Zhang,Min
title	Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
title_short	Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
title_full	Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
title_fullStr	Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
title_full_unstemmed	Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae
title_sort	modulation of mitochondrial membrane integrity and ros formation by high temperature in saccharomyces cerevisiae
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300010
work_keys_str_mv	AT zhangmin modulationofmitochondrialmembraneintegrityandrosformationbyhightemperatureinsaccharomycescerevisiae AT shijun modulationofmitochondrialmembraneintegrityandrosformationbyhightemperatureinsaccharomycescerevisiae AT jiangli modulationofmitochondrialmembraneintegrityandrosformationbyhightemperatureinsaccharomycescerevisiae
_version_	1718441909102313472

Modulation of mitochondrial membrane integrity and ROS formation by high temperature in Saccharomyces cerevisiae

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