mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep

mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep

Background The objective of this study was to compare the level differences of mRNA transcription and protein expression of PPARγ, FAS and HSL in different parts of the carcass in different tail-type sheep. Six Tan sheep and six Shaanbei fine-wool sheep aged 9 months were slaughtered and sa...

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Autores principales: Xu,Xiaochun, Wei,Xuan, Yang,Yuxin, Niu,Wenzhi, Kou,Qifang, Wang,Xiaolong, Chen,Yulin
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2015
Materias:
Genes
Difference
Tissues
Ovis
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300012
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spelling oai:scielo:S0717-345820150003000122015-06-26mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheepXu,XiaochunWei,XuanYang,YuxinNiu,WenzhiKou,QifangWang,XiaolongChen,Yulin Genes Difference Tissues Ovis Background The objective of this study was to compare the level differences of mRNA transcription and protein expression of PPARγ, FAS and HSL in different parts of the carcass in different tail-type sheep. Six Tan sheep and six Shaanbei fine-wool sheep aged 9 months were slaughtered and samples were collected from the tail adipose, subcutaneous adipose, and longissimus dorsi muscle. The levels of mRNA transcription and protein expression of the target genes in these tissues were determined by real-time quantitative PCR and western blot analyses. Results The results showed that PPARγ, FAS, and HSL were expressed with spatial differences in tail adipose, subcutaneous adipose and longissimus dorsi muscle of Tan sheep and Shaanbei fine-wool sheep. Differences were also observed between the two breeds. The mRNA transcription levels of these genes were somewhat consistent with their protein expression levels. Conclusion The present results indicated that PPARγ, FAS and HSL are correlated with fat deposition, especially for the regulating of adipose deposition in intramuscular fat, and that the mRNA expression patterns are similar to the protein expression patterns. The mechanism requires clarification in further studies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300012en10.1016/j.ejbt.2015.03.010
institution Scielo Chile
collection Scielo Chile
language English
topic Genes
Difference
Tissues
Ovis
spellingShingle Genes
Difference
Tissues
Ovis
Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
description Background The objective of this study was to compare the level differences of mRNA transcription and protein expression of PPARγ, FAS and HSL in different parts of the carcass in different tail-type sheep. Six Tan sheep and six Shaanbei fine-wool sheep aged 9 months were slaughtered and samples were collected from the tail adipose, subcutaneous adipose, and longissimus dorsi muscle. The levels of mRNA transcription and protein expression of the target genes in these tissues were determined by real-time quantitative PCR and western blot analyses. Results The results showed that PPARγ, FAS, and HSL were expressed with spatial differences in tail adipose, subcutaneous adipose and longissimus dorsi muscle of Tan sheep and Shaanbei fine-wool sheep. Differences were also observed between the two breeds. The mRNA transcription levels of these genes were somewhat consistent with their protein expression levels. Conclusion The present results indicated that PPARγ, FAS and HSL are correlated with fat deposition, especially for the regulating of adipose deposition in intramuscular fat, and that the mRNA expression patterns are similar to the protein expression patterns. The mechanism requires clarification in further studies.
author Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
author_facet Xu,Xiaochun
Wei,Xuan
Yang,Yuxin
Niu,Wenzhi
Kou,Qifang
Wang,Xiaolong
Chen,Yulin
author_sort Xu,Xiaochun
title mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
title_short mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
title_full mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
title_fullStr mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
title_full_unstemmed mRNA transcription and protein expression of PPARγ, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep
title_sort mrna transcription and protein expression of pparγ, fas, and hsl in different parts of the carcass between fat-tailed and thin-tailed sheep
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300012
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