Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium

Background Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett-Burman design (PBD) was applied to screen for significant factors, followed...

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Autores principales:	Mo,Jixian, Wang,Zhigang, Xu,Weihui, Li,Shanshan, Yu,Zhidan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Bacillus Box-Behnken design Dimethyl phthalate Medium optimization Plackett-Burman design
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300016
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spelling	oai:scielo:S0717-345820150003000162015-06-26Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation mediumMo,JixianWang,ZhigangXu,WeihuiLi,ShanshanYu,Zhidan Bacillus Box-Behnken design Dimethyl phthalate Medium optimization Plackett-Burman design Background Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett-Burman design (PBD) was applied to screen for significant factors, followed by the Steepest Ascent Method (SAM) to find the nearest region of maximum response. A Box-Behnken design (BBD) of the Response Surface Methodology (RSM) was conducted to optimize the final levels of the medium components. Results After the regression equation and response surface contour plots were analyzed, the concentrations of glucose, corn meal and NaCl were found to significantly influence the biomass of DMP-degrading bacteria. A combination of 22.88 g/L of glucose, 11.74 g/L of corn meal, and 10.34 g/L of NaCl was optimum for maximum biomass production of Bacillus sp. QD14. A 57.11% enhancement of the biomass production was gained after optimization in shake-flask cultivation. The biomass production of Bacillus sp. QD14 reached 9.13 ± 0.29 × 10(8) CFU/mL, which was an excellent match for the predicted value, and the mean value of the match degree was as high as 99.30%. Conclusion In this work, the key factors affected by the fermentation of DMP-degrading strain Bacillus sp. QD14 were optimized by PBD, SAM and BBD (RSM); the yield was increased by 57,11% in the conditions in our study. We propose that the conditions optimized in the study can be applied to the fermentation for commercialization production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.3 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300016en10.1016/j.ejbt.2015.03.013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus Box-Behnken design Dimethyl phthalate Medium optimization Plackett-Burman design
spellingShingle	Bacillus Box-Behnken design Dimethyl phthalate Medium optimization Plackett-Burman design Mo,Jixian Wang,Zhigang Xu,Weihui Li,Shanshan Yu,Zhidan Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
description	Background Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett-Burman design (PBD) was applied to screen for significant factors, followed by the Steepest Ascent Method (SAM) to find the nearest region of maximum response. A Box-Behnken design (BBD) of the Response Surface Methodology (RSM) was conducted to optimize the final levels of the medium components. Results After the regression equation and response surface contour plots were analyzed, the concentrations of glucose, corn meal and NaCl were found to significantly influence the biomass of DMP-degrading bacteria. A combination of 22.88 g/L of glucose, 11.74 g/L of corn meal, and 10.34 g/L of NaCl was optimum for maximum biomass production of Bacillus sp. QD14. A 57.11% enhancement of the biomass production was gained after optimization in shake-flask cultivation. The biomass production of Bacillus sp. QD14 reached 9.13 ± 0.29 × 10(8) CFU/mL, which was an excellent match for the predicted value, and the mean value of the match degree was as high as 99.30%. Conclusion In this work, the key factors affected by the fermentation of DMP-degrading strain Bacillus sp. QD14 were optimized by PBD, SAM and BBD (RSM); the yield was increased by 57,11% in the conditions in our study. We propose that the conditions optimized in the study can be applied to the fermentation for commercialization production.
author	Mo,Jixian Wang,Zhigang Xu,Weihui Li,Shanshan Yu,Zhidan
author_facet	Mo,Jixian Wang,Zhigang Xu,Weihui Li,Shanshan Yu,Zhidan
author_sort	Mo,Jixian
title	Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
title_short	Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
title_full	Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
title_fullStr	Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
title_full_unstemmed	Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium
title_sort	enhanced production of dimethyl phthalate-degrading strain bacillus sp. qd14 by optimizing fermentation medium
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300016
work_keys_str_mv	AT mojixian enhancedproductionofdimethylphthalatedegradingstrainbacillusspqd14byoptimizingfermentationmedium AT wangzhigang enhancedproductionofdimethylphthalatedegradingstrainbacillusspqd14byoptimizingfermentationmedium AT xuweihui enhancedproductionofdimethylphthalatedegradingstrainbacillusspqd14byoptimizingfermentationmedium AT lishanshan enhancedproductionofdimethylphthalatedegradingstrainbacillusspqd14byoptimizingfermentationmedium AT yuzhidan enhancedproductionofdimethylphthalatedegradingstrainbacillusspqd14byoptimizingfermentationmedium
_version_	1718441910794715136

Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium

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