Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus

Background Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed lacca...

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Autores principales:	Hernández,Christian A, Sandoval,Norma, Mallerman,Julieta, García-Pérez,José Antonio, Farnet,Anne-Marie, Perraud-Gaime,Isabelle, Alarcón,Enrique
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400012
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spelling	oai:scielo:S0717-345820150004000122015-12-04Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineusHernández,Christian ASandoval,NormaMallerman,JulietaGarcía-Pérez,José AntonioFarnet,Anne-MariePerraud-Gaime,IsabelleAlarcón,Enrique Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract Background Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available. Conclusions We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.4 20152015-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400012en10.1016/j.ejbt.2015.05.008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract
spellingShingle	Enzyme production Fungal metabolism Phenoloxidases Urea Yeast extract Hernández,Christian A Sandoval,Norma Mallerman,Julieta García-Pérez,José Antonio Farnet,Anne-Marie Perraud-Gaime,Isabelle Alarcón,Enrique Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
description	Background Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available. Conclusions We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.
author	Hernández,Christian A Sandoval,Norma Mallerman,Julieta García-Pérez,José Antonio Farnet,Anne-Marie Perraud-Gaime,Isabelle Alarcón,Enrique
author_facet	Hernández,Christian A Sandoval,Norma Mallerman,Julieta García-Pérez,José Antonio Farnet,Anne-Marie Perraud-Gaime,Isabelle Alarcón,Enrique
author_sort	Hernández,Christian A
title	Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
title_short	Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
title_full	Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
title_fullStr	Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
title_full_unstemmed	Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus
title_sort	ethanol induction of laccase depends on nitrogen conditions of pycnoporus sanguineus
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400012
work_keys_str_mv	AT hernandezchristiana ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT sandovalnorma ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT mallermanjulieta ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT garciaperezjoseantonio ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT farnetannemarie ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT perraudgaimeisabelle ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus AT alarconenrique ethanolinductionoflaccasedependsonnitrogenconditionsofpycnoporussanguineus
_version_	1718441914458439680

Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus

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