A simple and efficient method for extraction of Taq DNA polymerase
Background Thermostable DNA polymerase (Taq Pol ?) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method. The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. Moreover, we found that 30-40% activ...
Guardado en:
Autores principales: | Chen,Sique, Zheng,Xiujuan, Cao,Hongrui, Jiang,Linghui, Liu,Fangqian, Sun,Xinli |
---|---|
Lenguaje: | English |
Publicado: |
Pontificia Universidad Católica de Valparaíso
2015
|
Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500005 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Application of TaqMan Real-Time PCR for Detecting ‘<i>Candidatus</i> Arsenophonus Phytopathogenicus’ Infection in Sugar Beet
por: Christina Zübert, et al.
Publicado: (2021) -
Detección molecular de Dientamoeba fragilis en heces: eliminación de los inhibidores de la DNA polimerasa
por: MENGHI,CLAUDIA IRENE, et al.
Publicado: (2006) -
Development and Validation of Two Diagnostic Real-Time PCR (TaqMan) Assays for the Detection of <i>Bordetella avium</i> from Clinical Samples and Comparison to the Currently Available Real-Time TaqMan PCR Assay
por: Amro Hashish, et al.
Publicado: (2021) -
Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
por: Yu,Shu Tao, et al.
Publicado: (2010) -
Enhancing the specificity of polymerase chain reaction by graphene oxide through surface modification: zwitterionic polymer is superior to other polymers with different charges
por: Zhong Y, et al.
Publicado: (2016)