Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes

Background Several MYB genes belonging to R2R3 MYB transcription factors have been used in several plant species to enhance anthocyanin production, and have shown various expression or regulation patterns. This study focused on the effect of ectopic expression of an RsMYB1 isolated from radish (Raph...

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Autores principales:	Naing,Aung Htay, Ai,Trinh Ngoc, Jeon,Su Min, Park,Kyeung Il, Lim,Ki Byung, Kim,Chang Kil
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Anthocyanin Chrysanthemum Morphology RT- PCR Transcription factor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500006
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spelling	oai:scielo:S0717-345820150005000062016-03-21Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genesNaing,Aung HtayAi,Trinh NgocJeon,Su MinPark,Kyeung IlLim,Ki ByungKim,Chang Kil Anthocyanin Chrysanthemum Morphology RT- PCR Transcription factor Background Several MYB genes belonging to R2R3 MYB transcription factors have been used in several plant species to enhance anthocyanin production, and have shown various expression or regulation patterns. This study focused on the effect of ectopic expression of an RsMYB1 isolated from radish (Raphanus sativa) on chrysanthemum cv. Shinma'. Results The RT-PCR results confirmed that RsMYB1 regulated the expression of three key biosynthetic genes (CmF3H, CmDFR, and CmANS) that are responsible for anthocyanin production in transgenic chrysanthemum, but were not detected in the non-transgenic line. In all transgenic plants, higher expression levels of key biosynthetic genes were observed in flowers than in leaves. However, the presence of RsMYB1 in chrysanthemum did not affect any morphological characteristics, such as plant height, leaf shape or size, and number of flowers. Furthermore, no anthocyanin accumulation was visually observed in the leaves and floral tissue of any of the transgenic lines, which was further confirmed by anthocyanin content estimation. Conclusion To our knowledge, this is the first time the role of an MYB transcription factor in anthocyanin production has been investigated in chrysanthemum.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.5 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500006en10.1016/j.ejbt.2015.07.001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Anthocyanin Chrysanthemum Morphology RT- PCR Transcription factor
spellingShingle	Anthocyanin Chrysanthemum Morphology RT- PCR Transcription factor Naing,Aung Htay Ai,Trinh Ngoc Jeon,Su Min Park,Kyeung Il Lim,Ki Byung Kim,Chang Kil Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
description	Background Several MYB genes belonging to R2R3 MYB transcription factors have been used in several plant species to enhance anthocyanin production, and have shown various expression or regulation patterns. This study focused on the effect of ectopic expression of an RsMYB1 isolated from radish (Raphanus sativa) on chrysanthemum cv. Shinma'. Results The RT-PCR results confirmed that RsMYB1 regulated the expression of three key biosynthetic genes (CmF3H, CmDFR, and CmANS) that are responsible for anthocyanin production in transgenic chrysanthemum, but were not detected in the non-transgenic line. In all transgenic plants, higher expression levels of key biosynthetic genes were observed in flowers than in leaves. However, the presence of RsMYB1 in chrysanthemum did not affect any morphological characteristics, such as plant height, leaf shape or size, and number of flowers. Furthermore, no anthocyanin accumulation was visually observed in the leaves and floral tissue of any of the transgenic lines, which was further confirmed by anthocyanin content estimation. Conclusion To our knowledge, this is the first time the role of an MYB transcription factor in anthocyanin production has been investigated in chrysanthemum.
author	Naing,Aung Htay Ai,Trinh Ngoc Jeon,Su Min Park,Kyeung Il Lim,Ki Byung Kim,Chang Kil
author_facet	Naing,Aung Htay Ai,Trinh Ngoc Jeon,Su Min Park,Kyeung Il Lim,Ki Byung Kim,Chang Kil
author_sort	Naing,Aung Htay
title	Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
title_short	Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
title_full	Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
title_fullStr	Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
title_full_unstemmed	Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes
title_sort	expression of rsmyb1 in chrysanthemum regulates key anthocyanin biosynthetic genes
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500006
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Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes

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