Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa

Background Currently, the technology called Clearfield® is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acid...

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Autores principales:	Mestanza,Camilo, Riegel,Ricardo, Silva,Herman, Vásquez,Santiago C
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Acetohydroxyacid synthase Acetolactate synthase Chenopodium quinoa Homeologous pairing Gene duplication
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600002
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spelling	oai:scielo:S0717-345820150006000022016-01-21Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoaMestanza,CamiloRiegel,RicardoSilva,HermanVásquez,Santiago C Acetohydroxyacid synthase Acetolactate synthase Chenopodium quinoa Homeologous pairing Gene duplication Background Currently, the technology called Clearfield® is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acids valine, leucine, and isoleucine. Therefore, multiple copies of the AHAS gene might be of interest for breeding programs targeting herbicide resistance. In this work, the characterization of the AHAS gene was accomplished for the Chenopodium quinoa Regalona-Baer cultivar. Cloning, sequencing, and Southern blotting were conducted to determine the number of gene copies. Results The presence of multiple copies of the AHAS gene as has been shown previously in several other species is described. Six copies of the AHAS gene were confirmed with Southern blot analyses. CqHAS1 and CqAHAS2 variants showed the highest homology with AHAS mRNA sequences found in the NR Database. A third copy, CqAHAS3, shared similar fragments with both CqAHAS1 and CqAHAS2, suggesting duplication through homeologous chromosomes pairing. Conclusions The presence of multiple copies of the gene AHAS shows that gene duplication is a common feature in polyploid species during evolution. In addition, to our knowledge, this is the first report of the interaction of sub-genomes in quinoa.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600002en10.1016/j.ejbt.2015.07.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Acetohydroxyacid synthase Acetolactate synthase Chenopodium quinoa Homeologous pairing Gene duplication
spellingShingle	Acetohydroxyacid synthase Acetolactate synthase Chenopodium quinoa Homeologous pairing Gene duplication Mestanza,Camilo Riegel,Ricardo Silva,Herman Vásquez,Santiago C Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
description	Background Currently, the technology called Clearfield® is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acids valine, leucine, and isoleucine. Therefore, multiple copies of the AHAS gene might be of interest for breeding programs targeting herbicide resistance. In this work, the characterization of the AHAS gene was accomplished for the Chenopodium quinoa Regalona-Baer cultivar. Cloning, sequencing, and Southern blotting were conducted to determine the number of gene copies. Results The presence of multiple copies of the AHAS gene as has been shown previously in several other species is described. Six copies of the AHAS gene were confirmed with Southern blot analyses. CqHAS1 and CqAHAS2 variants showed the highest homology with AHAS mRNA sequences found in the NR Database. A third copy, CqAHAS3, shared similar fragments with both CqAHAS1 and CqAHAS2, suggesting duplication through homeologous chromosomes pairing. Conclusions The presence of multiple copies of the gene AHAS shows that gene duplication is a common feature in polyploid species during evolution. In addition, to our knowledge, this is the first report of the interaction of sub-genomes in quinoa.
author	Mestanza,Camilo Riegel,Ricardo Silva,Herman Vásquez,Santiago C
author_facet	Mestanza,Camilo Riegel,Ricardo Silva,Herman Vásquez,Santiago C
author_sort	Mestanza,Camilo
title	Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
title_short	Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
title_full	Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
title_fullStr	Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
title_full_unstemmed	Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa
title_sort	characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant chenopodium quinoa
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600002
work_keys_str_mv	AT mestanzacamilo characterizationoftheacetohydroxyacidsynthasemultigenefamilyinthetetraploideplantchenopodiumquinoa AT riegelricardo characterizationoftheacetohydroxyacidsynthasemultigenefamilyinthetetraploideplantchenopodiumquinoa AT silvaherman characterizationoftheacetohydroxyacidsynthasemultigenefamilyinthetetraploideplantchenopodiumquinoa AT vasquezsantiagoc characterizationoftheacetohydroxyacidsynthasemultigenefamilyinthetetraploideplantchenopodiumquinoa
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Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa

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