Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family

Background The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX pri...

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Autores principales:	Rampadarath,Sillma, Puchooa,Daneshwar, Bal,Subhasisa
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Euphorbiaceae Genetic diversity Jatropha Mitochondrial genome rep-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600005
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spelling	oai:scielo:S0717-345820150006000052016-01-21Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae familyRampadarath,SillmaPuchooa,DaneshwarBal,Subhasisa Euphorbiaceae Genetic diversity Jatropha Mitochondrial genome rep-PCR Background The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers. Results The agglomerative cluster analysis was used to evaluate the scoring data. BOX and REP gave amplification with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae. Both markers confirmed Jatropha tanjorensis as a natural hybrid between Jatropha gossypifolia and Jatropha curcas. Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results demonstrated that the amplified products were from the mitochondrial genomes. Conclusion The rep-PCR molecular tool can be used to characterize diversity in plants as they are suitable for distinguishing eukaryotic genomes effectively.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600005en10.1016/j.ejbt.2015.09.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Euphorbiaceae Genetic diversity Jatropha Mitochondrial genome rep-PCR
spellingShingle	Euphorbiaceae Genetic diversity Jatropha Mitochondrial genome rep-PCR Rampadarath,Sillma Puchooa,Daneshwar Bal,Subhasisa Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
description	Background The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers. Results The agglomerative cluster analysis was used to evaluate the scoring data. BOX and REP gave amplification with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae. Both markers confirmed Jatropha tanjorensis as a natural hybrid between Jatropha gossypifolia and Jatropha curcas. Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results demonstrated that the amplified products were from the mitochondrial genomes. Conclusion The rep-PCR molecular tool can be used to characterize diversity in plants as they are suitable for distinguishing eukaryotic genomes effectively.
author	Rampadarath,Sillma Puchooa,Daneshwar Bal,Subhasisa
author_facet	Rampadarath,Sillma Puchooa,Daneshwar Bal,Subhasisa
author_sort	Rampadarath,Sillma
title	Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
title_short	Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
title_full	Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
title_fullStr	Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
title_full_unstemmed	Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
title_sort	repetitive element palindromic pcr (rep-pcr) as a genetic tool to study interspecific diversity in euphorbiaceae family
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600005
work_keys_str_mv	AT rampadarathsillma repetitiveelementpalindromicpcrreppcrasagenetictooltostudyinterspecificdiversityineuphorbiaceaefamily AT puchooadaneshwar repetitiveelementpalindromicpcrreppcrasagenetictooltostudyinterspecificdiversityineuphorbiaceaefamily AT balsubhasisa repetitiveelementpalindromicpcrreppcrasagenetictooltostudyinterspecificdiversityineuphorbiaceaefamily
_version_	1718441919285035008

Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family

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