Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261

Background The effects of exposure to copper, during growth, on the production of biomass, total protein, catalase, glutathione-S transferase, glutathione peroxidase, peroxidase, polyphosphate, acid and alkaline phosphatases, ultrastructure and the ability to remove this metal from Aspergillus niger...

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Autores principales:	Cavalcanti Luna,Marcos A, Rodrigues Vieira,Edson, Okada,Kaoru, Campos-Takaki,Galba Maria, do Nascimento,Aline Elesbão
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Aspergillus niger Antioxidant enzyme Copper removal Polyphosphate Ultrastructure
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600006
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spelling	oai:scielo:S0717-345820150006000062016-01-21Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261Cavalcanti Luna,Marcos ARodrigues Vieira,EdsonOkada,KaoruCampos-Takaki,Galba Mariado Nascimento,Aline Elesbão Aspergillus niger Antioxidant enzyme Copper removal Polyphosphate Ultrastructure Background The effects of exposure to copper, during growth, on the production of biomass, total protein, catalase, glutathione-S transferase, glutathione peroxidase, peroxidase, polyphosphate, acid and alkaline phosphatases, ultrastructure and the ability to remove this metal from Aspergillus niger, obtained from caatinga soil, were evaluated. Results All parameters tested were influenced by the concentration of metal in the culture medium. The presence of metal induced high levels of antioxidant enzymes, including lipid peroxidation, thereby revealing the appearance of an oxidative stress response. The variation in polyphosphate levels indicates the participation of the polymer in response to stress induced by copper. The activities of the phosphatases were positively influenced by growing them in the presence of copper. Ultrastructure changes in the cell surface, electron density, thickness, and septation were visualized by exposing cells to increasingly larger concentrations of metal. The isolate was able to remove the agent from the growth medium, while maintaining its physiological functions. The metal removed from the cultures exposed to 0.5 mM, 1 mM and 2 mM copper exhibited percentages of removal equivalent to 75.78%, 66.04% and 33.51%. Conclusions The results indicate that the isolate was able to grow in high concentrations of copper, activates mechanisms for adaptation and tolerance in the presence of metal, and is highly efficient at removing the agent. Such data are fundamental if a better understanding is to be reached of the cellular and molecular abilities of native isolates, which can be used to develop bioprocesses in environmental and industrial areas.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600006en10.1016/j.ejbt.2015.09.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aspergillus niger Antioxidant enzyme Copper removal Polyphosphate Ultrastructure
spellingShingle	Aspergillus niger Antioxidant enzyme Copper removal Polyphosphate Ultrastructure Cavalcanti Luna,Marcos A Rodrigues Vieira,Edson Okada,Kaoru Campos-Takaki,Galba Maria do Nascimento,Aline Elesbão Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
description	Background The effects of exposure to copper, during growth, on the production of biomass, total protein, catalase, glutathione-S transferase, glutathione peroxidase, peroxidase, polyphosphate, acid and alkaline phosphatases, ultrastructure and the ability to remove this metal from Aspergillus niger, obtained from caatinga soil, were evaluated. Results All parameters tested were influenced by the concentration of metal in the culture medium. The presence of metal induced high levels of antioxidant enzymes, including lipid peroxidation, thereby revealing the appearance of an oxidative stress response. The variation in polyphosphate levels indicates the participation of the polymer in response to stress induced by copper. The activities of the phosphatases were positively influenced by growing them in the presence of copper. Ultrastructure changes in the cell surface, electron density, thickness, and septation were visualized by exposing cells to increasingly larger concentrations of metal. The isolate was able to remove the agent from the growth medium, while maintaining its physiological functions. The metal removed from the cultures exposed to 0.5 mM, 1 mM and 2 mM copper exhibited percentages of removal equivalent to 75.78%, 66.04% and 33.51%. Conclusions The results indicate that the isolate was able to grow in high concentrations of copper, activates mechanisms for adaptation and tolerance in the presence of metal, and is highly efficient at removing the agent. Such data are fundamental if a better understanding is to be reached of the cellular and molecular abilities of native isolates, which can be used to develop bioprocesses in environmental and industrial areas.
author	Cavalcanti Luna,Marcos A Rodrigues Vieira,Edson Okada,Kaoru Campos-Takaki,Galba Maria do Nascimento,Aline Elesbão
author_facet	Cavalcanti Luna,Marcos A Rodrigues Vieira,Edson Okada,Kaoru Campos-Takaki,Galba Maria do Nascimento,Aline Elesbão
author_sort	Cavalcanti Luna,Marcos A
title	Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
title_short	Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
title_full	Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
title_fullStr	Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
title_full_unstemmed	Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261
title_sort	copper-induced adaptation, oxidative stress and its tolerance in aspergillus niger ucp1261
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600006
work_keys_str_mv	AT cavalcantilunamarcosa copperinducedadaptationoxidativestressanditstoleranceinaspergillusnigerucp1261 AT rodriguesvieiraedson copperinducedadaptationoxidativestressanditstoleranceinaspergillusnigerucp1261 AT okadakaoru copperinducedadaptationoxidativestressanditstoleranceinaspergillusnigerucp1261 AT campostakakigalbamaria copperinducedadaptationoxidativestressanditstoleranceinaspergillusnigerucp1261 AT donascimentoalineelesbao copperinducedadaptationoxidativestressanditstoleranceinaspergillusnigerucp1261
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Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261

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