Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran

Background Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results Among the 12 clones st...

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Autores principales:	Azadi Gonbad,Reza, Afzan,Adlin, Karimi,Ehsan, Sinniah,Uma Rani, Kumara Swamy,Mallappa
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Camellia sinensis DPPH assay Methanolic extract Total flavonoid content Total phenolic content
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600008
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spelling	oai:scielo:S0717-345820150006000082016-01-21Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of IranAzadi Gonbad,RezaAfzan,AdlinKarimi,EhsanSinniah,Uma RaniKumara Swamy,Mallappa Camellia sinensis DPPH assay Methanolic extract Total flavonoid content Total phenolic content Background Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results Among the 12 clones studied, tea clone Iran 100 had the highest total phenolic content and total flavonoid content with values of 8.44 ± 1.03 mg gallic acid equivalents per gram dry weight and 4.50 ± 0.16 mg rutin equivalents per gram dry weight respectively. High performance Liquid Chromatography (HPLC) analysis of phenolics and flavonoids in 12 clones revealed the presence of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epigallocatechin-gallate, (-)-epicatechingallate, gallic acid and caffeine. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay showed the existence of variation in the antioxidant activity ranging from 22.67 to 65.36%. The highest antioxidant activity with IC50 value of 218.24 µg/mL was observed in the leaf extract of the clone Iran 100, while the lowest was found in the clone Iran 482 with IC50 value of 234.44 µg/mL. The antioxidant activity had a positive correlation with total phenolic content, total flavonoid content, (-)-epigallocatechin-gallate, (-)-epicatechingallate and caffeine (0.59 = r = 0.97, P < 0.05). Conclusion From the study it can be concluded that the clone Iran 100 has a superior quality compared to any other clones studied due to occurrence of more phenolic compounds and a greater antioxidant activity. Hence, we recommend the use of tea clone Iran 100 for commercial planting.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600008en10.1016/j.ejbt.2015.08.007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Camellia sinensis DPPH assay Methanolic extract Total flavonoid content Total phenolic content
spellingShingle	Camellia sinensis DPPH assay Methanolic extract Total flavonoid content Total phenolic content Azadi Gonbad,Reza Afzan,Adlin Karimi,Ehsan Sinniah,Uma Rani Kumara Swamy,Mallappa Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
description	Background Tea (Camellia sinensis), a well-known beverage is consumed frequently worldwide due to its high antioxidant properties. The present study determines the amount of phytochemicals and antioxidant activities among 12 high yielding tea clones cultivated in Iran. Results Among the 12 clones studied, tea clone Iran 100 had the highest total phenolic content and total flavonoid content with values of 8.44 ± 1.03 mg gallic acid equivalents per gram dry weight and 4.50 ± 0.16 mg rutin equivalents per gram dry weight respectively. High performance Liquid Chromatography (HPLC) analysis of phenolics and flavonoids in 12 clones revealed the presence of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epigallocatechin-gallate, (-)-epicatechingallate, gallic acid and caffeine. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay showed the existence of variation in the antioxidant activity ranging from 22.67 to 65.36%. The highest antioxidant activity with IC50 value of 218.24 µg/mL was observed in the leaf extract of the clone Iran 100, while the lowest was found in the clone Iran 482 with IC50 value of 234.44 µg/mL. The antioxidant activity had a positive correlation with total phenolic content, total flavonoid content, (-)-epigallocatechin-gallate, (-)-epicatechingallate and caffeine (0.59 = r = 0.97, P < 0.05). Conclusion From the study it can be concluded that the clone Iran 100 has a superior quality compared to any other clones studied due to occurrence of more phenolic compounds and a greater antioxidant activity. Hence, we recommend the use of tea clone Iran 100 for commercial planting.
author	Azadi Gonbad,Reza Afzan,Adlin Karimi,Ehsan Sinniah,Uma Rani Kumara Swamy,Mallappa
author_facet	Azadi Gonbad,Reza Afzan,Adlin Karimi,Ehsan Sinniah,Uma Rani Kumara Swamy,Mallappa
author_sort	Azadi Gonbad,Reza
title	Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_short	Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_full	Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_fullStr	Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_full_unstemmed	Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran
title_sort	phytoconstituents and antioxidant properties among commercial tea (camellia sinensis l.) clones of iran
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600008
work_keys_str_mv	AT azadigonbadreza phytoconstituentsandantioxidantpropertiesamongcommercialteacamelliasinensislclonesofiran AT afzanadlin phytoconstituentsandantioxidantpropertiesamongcommercialteacamelliasinensislclonesofiran AT karimiehsan phytoconstituentsandantioxidantpropertiesamongcommercialteacamelliasinensislclonesofiran AT sinniahumarani phytoconstituentsandantioxidantpropertiesamongcommercialteacamelliasinensislclonesofiran AT kumaraswamymallappa phytoconstituentsandantioxidantpropertiesamongcommercialteacamelliasinensislclonesofiran
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Phytoconstituents and antioxidant properties among commercial tea (Camellia sinensis L.) clones of Iran

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