Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi

Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered wh...

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Autores principales:	Lourdes Rocha,Carla Maria, Vellicce,Gabriel Ricardo, García,María Gabriela, Pardo,Esteban Mariano, Racedo,Josefina, Perera,María Francisca, de Lucía,Adrian, Gilli,Javier, Bogado,Noelia, Bonnecarrère,Victoria, German,Silvia, Marcelino,Francismar, Ledesma,Fernando, Reznikov,Sebastián, Ploper,Leonardo Daniel, Welin,Bjorn, Castagnaro,Atilio Pedro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Asian soybean rust Genetic variation Glycine max Molecular markers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009
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spelling	oai:scielo:S0717-345820150006000092016-01-21Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhiziLourdes Rocha,Carla MariaVellicce,Gabriel RicardoGarcía,María GabrielaPardo,Esteban MarianoRacedo,JosefinaPerera,María Franciscade Lucía,AdrianGilli,JavierBogado,NoeliaBonnecarrère,VictoriaGerman,SilviaMarcelino,FrancismarLedesma,FernandoReznikov,SebastiánPloper,Leonardo DanielWelin,BjornCastagnaro,Atilio Pedro Asian soybean rust Genetic variation Glycine max Molecular markers Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009en10.1016/j.ejbt.2015.06.007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Asian soybean rust Genetic variation Glycine max Molecular markers
spellingShingle	Asian soybean rust Genetic variation Glycine max Molecular markers Lourdes Rocha,Carla Maria Vellicce,Gabriel Ricardo García,María Gabriela Pardo,Esteban Mariano Racedo,Josefina Perera,María Francisca de Lucía,Adrian Gilli,Javier Bogado,Noelia Bonnecarrère,Victoria German,Silvia Marcelino,Francismar Ledesma,Fernando Reznikov,Sebastián Ploper,Leonardo Daniel Welin,Bjorn Castagnaro,Atilio Pedro Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
description	Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
author	Lourdes Rocha,Carla Maria Vellicce,Gabriel Ricardo García,María Gabriela Pardo,Esteban Mariano Racedo,Josefina Perera,María Francisca de Lucía,Adrian Gilli,Javier Bogado,Noelia Bonnecarrère,Victoria German,Silvia Marcelino,Francismar Ledesma,Fernando Reznikov,Sebastián Ploper,Leonardo Daniel Welin,Bjorn Castagnaro,Atilio Pedro
author_facet	Lourdes Rocha,Carla Maria Vellicce,Gabriel Ricardo García,María Gabriela Pardo,Esteban Mariano Racedo,Josefina Perera,María Francisca de Lucía,Adrian Gilli,Javier Bogado,Noelia Bonnecarrère,Victoria German,Silvia Marcelino,Francismar Ledesma,Fernando Reznikov,Sebastián Ploper,Leonardo Daniel Welin,Bjorn Castagnaro,Atilio Pedro
author_sort	Lourdes Rocha,Carla Maria
title	Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
title_short	Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
title_full	Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
title_fullStr	Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
title_full_unstemmed	Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
title_sort	use of aflp markers to estimate molecular diversity of phakopsora pachyrhizi
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009
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Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi

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