Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12

Background Osmolytes with their effective stabilizing properties are accumulated as protectants not only against salinity but also against denaturing harsh environmental stresses such as freezing, drying, high temperatures, oxygen radicals and radiation. The present work seeks to understand how Halo...

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Autores principales:	Mangaoglu Yoruk,Hilal, Alpagu Sayar,Nihat
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2015
Materias:	Flux balance analysis Flux distribution Halomonas sp. AAD12 Halophilic Principle component analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600015
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spelling	oai:scielo:S0717-345820150006000152016-01-21Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12Mangaoglu Yoruk,HilalAlpagu Sayar,Nihat Flux balance analysis Flux distribution Halomonas sp. AAD12 Halophilic Principle component analysis Background Osmolytes with their effective stabilizing properties are accumulated as protectants not only against salinity but also against denaturing harsh environmental stresses such as freezing, drying, high temperatures, oxygen radicals and radiation. The present work seeks to understand how Halomonas sp. AAD12 cells redirect carbon flux specifically to replenish reactions for biomass and osmolyte synthesis under changing salinity and temperature. To accomplish this goal, a combined FBA-PCA approach has been utilized. Results Experimental data were collected to supply model constraints for FBA and for the verification of the model predictions, which were satisfactory. With restrictions on the various combinations of selected anaplerotic paths (reactions catalyzed by phosphoenolpyruvate carboxylase, pyruvate carboxylase or glyoxylate shunt), two major phenotypes were found. Moreover, under high salt concentrations, when the glucose uptake rate was over 1.1 mmoL DCW- 1 h- 1, an overflow metabolism that led to the synthesis of ethanol caused a slight change in both phenotypes. Conclusions The operation of the glyoxylate shunt as the major anaplerotic pathway and the degradation of 6-phosphogluconate through the Entner-Doudoroff Pathway were the major factors in causing a distinction between the observed phenotypes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.18 n.6 20152015-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600015en10.1016/j.ejbt.2015.09.010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Flux balance analysis Flux distribution Halomonas sp. AAD12 Halophilic Principle component analysis
spellingShingle	Flux balance analysis Flux distribution Halomonas sp. AAD12 Halophilic Principle component analysis Mangaoglu Yoruk,Hilal Alpagu Sayar,Nihat Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
description	Background Osmolytes with their effective stabilizing properties are accumulated as protectants not only against salinity but also against denaturing harsh environmental stresses such as freezing, drying, high temperatures, oxygen radicals and radiation. The present work seeks to understand how Halomonas sp. AAD12 cells redirect carbon flux specifically to replenish reactions for biomass and osmolyte synthesis under changing salinity and temperature. To accomplish this goal, a combined FBA-PCA approach has been utilized. Results Experimental data were collected to supply model constraints for FBA and for the verification of the model predictions, which were satisfactory. With restrictions on the various combinations of selected anaplerotic paths (reactions catalyzed by phosphoenolpyruvate carboxylase, pyruvate carboxylase or glyoxylate shunt), two major phenotypes were found. Moreover, under high salt concentrations, when the glucose uptake rate was over 1.1 mmoL DCW- 1 h- 1, an overflow metabolism that led to the synthesis of ethanol caused a slight change in both phenotypes. Conclusions The operation of the glyoxylate shunt as the major anaplerotic pathway and the degradation of 6-phosphogluconate through the Entner-Doudoroff Pathway were the major factors in causing a distinction between the observed phenotypes.
author	Mangaoglu Yoruk,Hilal Alpagu Sayar,Nihat
author_facet	Mangaoglu Yoruk,Hilal Alpagu Sayar,Nihat
author_sort	Mangaoglu Yoruk,Hilal
title	Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
title_short	Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
title_full	Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
title_fullStr	Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
title_full_unstemmed	Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12
title_sort	topological analysis of carbon flux during multi-stress adaptation in halomonas sp. aad12
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600015
work_keys_str_mv	AT mangaogluyorukhilal topologicalanalysisofcarbonfluxduringmultistressadaptationinhalomonasspaad12 AT alpagusayarnihat topologicalanalysisofcarbonfluxduringmultistressadaptationinhalomonasspaad12
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Topological analysis of carbon flux during multi-stress adaptation in Halomonas sp. AAD12

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