Three new shuttle vectors for heterologous expression in Zymomonas mobilis

Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first con...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cao,Qinghua, Li,Tao, Shao,Huanhuan, Tan,Xuemei, Zhang,Yizheng
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Expression vector Gene expression Glucoamylase Zymomonas mobilis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582016000100006
record_format	dspace
spelling	oai:scielo:S0717-345820160001000062016-05-11Three new shuttle vectors for heterologous expression in Zymomonas mobilisCao,QinghuaLi,TaoShao,HuanhuanTan,XuemeiZhang,Yizheng Expression vector Gene expression Glucoamylase Zymomonas mobilis Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first constructed with the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectors were stable in Z. mobilis ZM4 and have 3,32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene, was clonedinto the shuttle vectors, generatingthe expressionvectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori combined with the signal peptide sequence from the alkaline phosphatase gene of Z. mobilis was cloned into pSUZM1(2, 3)a, resulting in the plasmids pSUZM1a-GA, pSUZM2a-GA and pSUZM3a-GA, respectively. After transforming these plasmids into Z. mobilis ZM4, the host was endowed with glucoamylase activity for starch hydrolysis. Both pSUZM2a-GA and pSUZM3a-GA were more efficientatproducingglucoamylase thanpSUZM1a-GA. Conclusions: These results indicated that these expression vectors are useful tools for gene expression in Z. mobilis and this could provide a solid foundation for further studies of heterologous gene expression in Z. mobilis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.1 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100006en10.1016/j.ejbt.2015.11.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Expression vector Gene expression Glucoamylase Zymomonas mobilis
spellingShingle	Expression vector Gene expression Glucoamylase Zymomonas mobilis Cao,Qinghua Li,Tao Shao,Huanhuan Tan,Xuemei Zhang,Yizheng Three new shuttle vectors for heterologous expression in Zymomonas mobilis
description	Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first constructed with the origins of replication from the chromosome and two native plasmids (pZZM401 and pZZM402) of Z. mobilis ZM4, respectively. The three shuttle vectors were stable in Z. mobilis ZM4 and have 3,32 and 27 copies, respectively. The promoter Ppdc (a), from the pyruvate decarboxylase gene, was clonedinto the shuttle vectors, generatingthe expressionvectors pSUZM1(2, 3)a. The codon-optimized glucoamylase gene from Aspergillus awamori combined with the signal peptide sequence from the alkaline phosphatase gene of Z. mobilis was cloned into pSUZM1(2, 3)a, resulting in the plasmids pSUZM1a-GA, pSUZM2a-GA and pSUZM3a-GA, respectively. After transforming these plasmids into Z. mobilis ZM4, the host was endowed with glucoamylase activity for starch hydrolysis. Both pSUZM2a-GA and pSUZM3a-GA were more efficientatproducingglucoamylase thanpSUZM1a-GA. Conclusions: These results indicated that these expression vectors are useful tools for gene expression in Z. mobilis and this could provide a solid foundation for further studies of heterologous gene expression in Z. mobilis.
author	Cao,Qinghua Li,Tao Shao,Huanhuan Tan,Xuemei Zhang,Yizheng
author_facet	Cao,Qinghua Li,Tao Shao,Huanhuan Tan,Xuemei Zhang,Yizheng
author_sort	Cao,Qinghua
title	Three new shuttle vectors for heterologous expression in Zymomonas mobilis
title_short	Three new shuttle vectors for heterologous expression in Zymomonas mobilis
title_full	Three new shuttle vectors for heterologous expression in Zymomonas mobilis
title_fullStr	Three new shuttle vectors for heterologous expression in Zymomonas mobilis
title_full_unstemmed	Three new shuttle vectors for heterologous expression in Zymomonas mobilis
title_sort	three new shuttle vectors for heterologous expression in zymomonas mobilis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100006
work_keys_str_mv	AT caoqinghua threenewshuttlevectorsforheterologousexpressioninzymomonasmobilis AT litao threenewshuttlevectorsforheterologousexpressioninzymomonasmobilis AT shaohuanhuan threenewshuttlevectorsforheterologousexpressioninzymomonasmobilis AT tanxuemei threenewshuttlevectorsforheterologousexpressioninzymomonasmobilis AT zhangyizheng threenewshuttlevectorsforheterologousexpressioninzymomonasmobilis
_version_	1718441923892477952

Three new shuttle vectors for heterologous expression in Zymomonas mobilis

Ejemplares similares