Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1

Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% an...

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Autores principales:	Zhanga,Yong, Min,Qingsong, Xu,Jian, Zhang,Ke, Chen,Shulin, Wang,Haijun, Li,Demao
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	DHA Malate NADPH Schizochytrium sp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100009
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spelling	oai:scielo:S0717-345820160001000092016-05-11Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1Zhanga,YongMin,QingsongXu,JianZhang,KeChen,ShulinWang,HaijunLi,Demao DHA Malate NADPH Schizochytrium sp Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specificcell growth rate(μ), specific glucose consumption rate (qGlu)and DHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7 g/L, giving a yield of 0.103 g DHA/g glucose and a productivity of 284 mg L-1 h-1. Conclusion: A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applicationsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.1 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100009en10.1016/j.ejbt.2015.11.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DHA Malate NADPH Schizochytrium sp
spellingShingle	DHA Malate NADPH Schizochytrium sp Zhanga,Yong Min,Qingsong Xu,Jian Zhang,Ke Chen,Shulin Wang,Haijun Li,Demao Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
description	Background: Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. Results: DHA production increased by 47% and reached 5.51 g/L when 4 g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specificcell growth rate(μ), specific glucose consumption rate (qGlu)and DHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7 g/L, giving a yield of 0.103 g DHA/g glucose and a productivity of 284 mg L-1 h-1. Conclusion: A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applications
author	Zhanga,Yong Min,Qingsong Xu,Jian Zhang,Ke Chen,Shulin Wang,Haijun Li,Demao
author_facet	Zhanga,Yong Min,Qingsong Xu,Jian Zhang,Ke Chen,Shulin Wang,Haijun Li,Demao
author_sort	Zhanga,Yong
title	Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
title_short	Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
title_full	Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
title_fullStr	Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
title_full_unstemmed	Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
title_sort	effect of malate on docosahexaenoic acid production from schizochytrium sp. b4d1
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100009
work_keys_str_mv	AT zhangayong effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT minqingsong effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT xujian effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT zhangke effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT chenshulin effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT wanghaijun effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1 AT lidemao effectofmalateondocosahexaenoicacidproductionfromschizochytriumspb4d1
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Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1

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