Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment

Background: A study of the correlation between the particle size of lignocellulosic substrates and ultrasound pretreatment on the efficiency of further enzymatic hydrolysis and fermentation to ethanol. Results: The maximum concentrations of glucose and, to a lesser extent, di- and trisaccharides wer...

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Autores principales:	Revin,Victor, Atykyan,Nelli, Zakharkin,Denis
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Bioethanol Ligsnocelluloses Yeast Saccharomyces cerevisiae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200003
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spelling	oai:scielo:S0717-345820160002000032016-05-17Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatmentRevin,VictorAtykyan,NelliZakharkin,Denis Bioethanol Ligsnocelluloses Yeast Saccharomyces cerevisiae Background: A study of the correlation between the particle size of lignocellulosic substrates and ultrasound pretreatment on the efficiency of further enzymatic hydrolysis and fermentation to ethanol. Results: The maximum concentrations of glucose and, to a lesser extent, di- and trisaccharides were obtained in a series of experiments with 48-h enzymatic hydrolysis of pine raw materials ground at 380-100 rpm for 30 min. The highest glucose yield was observed at the end of the hydrolysis with a cellulase dosage of 10 mg of protein (204 ±21 units CMCase per g of sawdust). The greatest enzymatic hydrolysis efficiency was observed in a sample that combined two-stage grinding at 400 rpm with ultrasonic treatment for 5-10 min at a power of 10 W per kg of sawdust. The glucose yield in this case (35.5 g glucose l-1) increased twofold compared to ground substrate without further preparation. Conclusions: Using a mechanical two-stage grinding of lignocellulosic raw materials with ultrasonication increases the efficiency of subsequent enzymatic hydrolysis and fermentation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.2 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200003en10.1016/j.ejbt.2015.11.007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bioethanol Ligsnocelluloses Yeast Saccharomyces cerevisiae
spellingShingle	Bioethanol Ligsnocelluloses Yeast Saccharomyces cerevisiae Revin,Victor Atykyan,Nelli Zakharkin,Denis Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
description	Background: A study of the correlation between the particle size of lignocellulosic substrates and ultrasound pretreatment on the efficiency of further enzymatic hydrolysis and fermentation to ethanol. Results: The maximum concentrations of glucose and, to a lesser extent, di- and trisaccharides were obtained in a series of experiments with 48-h enzymatic hydrolysis of pine raw materials ground at 380-100 rpm for 30 min. The highest glucose yield was observed at the end of the hydrolysis with a cellulase dosage of 10 mg of protein (204 ±21 units CMCase per g of sawdust). The greatest enzymatic hydrolysis efficiency was observed in a sample that combined two-stage grinding at 400 rpm with ultrasonic treatment for 5-10 min at a power of 10 W per kg of sawdust. The glucose yield in this case (35.5 g glucose l-1) increased twofold compared to ground substrate without further preparation. Conclusions: Using a mechanical two-stage grinding of lignocellulosic raw materials with ultrasonication increases the efficiency of subsequent enzymatic hydrolysis and fermentation.
author	Revin,Victor Atykyan,Nelli Zakharkin,Denis
author_facet	Revin,Victor Atykyan,Nelli Zakharkin,Denis
author_sort	Revin,Victor
title	Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
title_short	Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
title_full	Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
title_fullStr	Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
title_full_unstemmed	Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
title_sort	enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200003
work_keys_str_mv	AT revinvictor enzymatichydrolysisandfermentationofultradispersedwoodparticlesafterultrasonicpretreatment AT atykyannelli enzymatichydrolysisandfermentationofultradispersedwoodparticlesafterultrasonicpretreatment AT zakharkindenis enzymatichydrolysisandfermentationofultradispersedwoodparticlesafterultrasonicpretreatment
_version_	1718441925850169344

Enzymatic hydrolysis and fermentation of ultradispersed wood particles after ultrasonic pretreatment

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