Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum

Background: Endophytic bacteria are ubiquitous in all plant species contributing in host plant's nutrient uptake and helping the host to improve its growth. Moringa peregrina which is a medicinal plant, growing in arid region of Arabia, was assessed for the presence of endophytic bacterial stra...

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Autores principales:	Latif Khan,Abdul, Ahmed Halo,Boshra, Elyassi,Ali, Ali,Sajid, Al-Hosni,Khadija, Hussain,Javid, Al-Harrasi,Ahmed, Lee,In-Jung
Lenguaje:	Spanish / Castilian
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	ACC deaminase Bacillus subtilis LK14 Endophytism Indole 3-acetic acid Tomato plant growth
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300007
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spelling	oai:scielo:S0717-345820160003000072016-07-06Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicumLatif Khan,AbdulAhmed Halo,BoshraElyassi,AliAli,SajidAl-Hosni,KhadijaHussain,JavidAl-Harrasi,AhmedLee,In-Jung ACC deaminase Bacillus subtilis LK14 Endophytism Indole 3-acetic acid Tomato plant growth Background: Endophytic bacteria are ubiquitous in all plant species contributing in host plant's nutrient uptake and helping the host to improve its growth. Moringa peregrina which is a medicinal plant, growing in arid region of Arabia, was assessed for the presence of endophytic bacterial strains. Results: PCR amplification and sequencing of 16S rRNA of bacterial endophytes revealed the 5 endophytic bacteria, in which 2 strains were from Sphingomonas sp.; 2 strains from Bacillus sp. and 1 from Methylobacterium genus. Among the endophytic bacterial strains, a strain of Bacillus subtilis LK14 has shown significant prospects in phosphate solubilization (clearing zone of 56.71 mm after 5 d), ACC deaminase (448.3 ± 2.91 nM α-ketobutyrate mg-1 h-1) and acid phosphatase activity (8.4 ± 1.2 nM mg-1 min-1). The endophytic bacteria were also assessed for their potential to produce indole-3-acetic acid (IAA). Among isolated strains, the initial spectrophotometry analysis showed significantly higher IAA production by Bacillus subtilis LK14. The diurnal production of IAA was quantified using multiple reactions monitoring method in UPLC/MS-MS. The analysis showed that LK14 produced the highest (8.7 uM) IAA on 14th d of growth. Looking at LK14 potentials, it was applied to Solanum lycopersicum, where it significantly increased the shoot and root biomass and chlorophyll (a and b) contents as compared to control plants. Conclusion: The study concludes that using endophytic bacterial strains can be bio-prospective for plant growth promotion, which might be an ideal strategy for improving growth of crops in marginal lands.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.3 20162016-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300007es10.1016/j.ejbt.2016.02.001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	ACC deaminase Bacillus subtilis LK14 Endophytism Indole 3-acetic acid Tomato plant growth
spellingShingle	ACC deaminase Bacillus subtilis LK14 Endophytism Indole 3-acetic acid Tomato plant growth Latif Khan,Abdul Ahmed Halo,Boshra Elyassi,Ali Ali,Sajid Al-Hosni,Khadija Hussain,Javid Al-Harrasi,Ahmed Lee,In-Jung Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
description	Background: Endophytic bacteria are ubiquitous in all plant species contributing in host plant's nutrient uptake and helping the host to improve its growth. Moringa peregrina which is a medicinal plant, growing in arid region of Arabia, was assessed for the presence of endophytic bacterial strains. Results: PCR amplification and sequencing of 16S rRNA of bacterial endophytes revealed the 5 endophytic bacteria, in which 2 strains were from Sphingomonas sp.; 2 strains from Bacillus sp. and 1 from Methylobacterium genus. Among the endophytic bacterial strains, a strain of Bacillus subtilis LK14 has shown significant prospects in phosphate solubilization (clearing zone of 56.71 mm after 5 d), ACC deaminase (448.3 ± 2.91 nM α-ketobutyrate mg-1 h-1) and acid phosphatase activity (8.4 ± 1.2 nM mg-1 min-1). The endophytic bacteria were also assessed for their potential to produce indole-3-acetic acid (IAA). Among isolated strains, the initial spectrophotometry analysis showed significantly higher IAA production by Bacillus subtilis LK14. The diurnal production of IAA was quantified using multiple reactions monitoring method in UPLC/MS-MS. The analysis showed that LK14 produced the highest (8.7 uM) IAA on 14th d of growth. Looking at LK14 potentials, it was applied to Solanum lycopersicum, where it significantly increased the shoot and root biomass and chlorophyll (a and b) contents as compared to control plants. Conclusion: The study concludes that using endophytic bacterial strains can be bio-prospective for plant growth promotion, which might be an ideal strategy for improving growth of crops in marginal lands.
author	Latif Khan,Abdul Ahmed Halo,Boshra Elyassi,Ali Ali,Sajid Al-Hosni,Khadija Hussain,Javid Al-Harrasi,Ahmed Lee,In-Jung
author_facet	Latif Khan,Abdul Ahmed Halo,Boshra Elyassi,Ali Ali,Sajid Al-Hosni,Khadija Hussain,Javid Al-Harrasi,Ahmed Lee,In-Jung
author_sort	Latif Khan,Abdul
title	Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
title_short	Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
title_full	Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
title_fullStr	Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
title_full_unstemmed	Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum
title_sort	indole acetic acid and acc deaminase from endophytic bacteria improves the growth of solarium lycopersicum
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300007
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_version_	1718441928455880704

Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solarium lycopersicum

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