Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing
Background: Wheat 1BL/1RS translocation lines are planted around the world for their disease resistance and high yield. Most of them are poor in bread making, which is partially caused by ω-secalins that are encoded by the ω-secalin gene family, which is located on the short arm of...
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Pontificia Universidad Católica de Valparaíso
2016
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oai:scielo:S0717-345820160003000102016-07-06Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencingLi,Meng JunLi,Ya QingZhang,NanShi,Zhan Liang Bacterial artificial chromosome (BAC) Wheat ω-Secalin 1BL/1RS translocation line Background: Wheat 1BL/1RS translocation lines are planted around the world for their disease resistance and high yield. Most of them are poor in bread making, which is partially caused by ω-secalins that are encoded by the ω-secalin gene family, which is located on the short arm of rye chromosome 1R (1RS). However, information on the structure and evolution of the ω-secalin gene family is still limited. Results: We first generated a physical map of the ω-secalin gene family covering 195 kb of the Sec-1 locus based on sequencing three bacterial artificial chromosome (BAC) clones of the 1BL/1RS translocation wheat cultivar Shimai 15. A BAC contig was constructed spanning 168 kb of the Sec-1 locus on 1RS. Twelve ω-secalin genes were arranged in a head-to-tail fashion, separated by 8.2-21.6 kb spacers on the contig, whereas six other ω-secalin genes were arranged head-to-tail, separated by 8.2-8.4 kb of spacers on clone BAC125. The 18 ω-secalin genes can be classified into six types among which eight ω-secalin genes were expressed during seed development. The ω-secalin genes with the 1074-bp open reading frame (ORF) represented the main population. Except for two pseudogenes, the N-terminal of the ω-secalin gene was conserved, whereas variations in the C-terminal led to a change in ORF length. The spacers can be sorted into two classes. Class-1 spacers contained conserved and non-conservative sequences. Conclusion: The ω-secalin gene family consisted of at least 18 members in the 1BL/1RS translocation line cv. Shimai 15. Eight ω-secalin genes were expressed during seed development. Eighteen members may originate from a progenitor with a 1,074-bp ORF. The spacers differed in length and sequence conservation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.3 20162016-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300010en10.1016/j.ejbt.2015.12.001 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Bacterial artificial chromosome (BAC) Wheat ω-Secalin 1BL/1RS translocation line |
| spellingShingle |
Bacterial artificial chromosome (BAC) Wheat ω-Secalin 1BL/1RS translocation line Li,Meng Jun Li,Ya Qing Zhang,Nan Shi,Zhan Liang Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| description |
Background: Wheat 1BL/1RS translocation lines are planted around the world for their disease resistance and high yield. Most of them are poor in bread making, which is partially caused by ω-secalins that are encoded by the ω-secalin gene family, which is located on the short arm of rye chromosome 1R (1RS). However, information on the structure and evolution of the ω-secalin gene family is still limited. Results: We first generated a physical map of the ω-secalin gene family covering 195 kb of the Sec-1 locus based on sequencing three bacterial artificial chromosome (BAC) clones of the 1BL/1RS translocation wheat cultivar Shimai 15. A BAC contig was constructed spanning 168 kb of the Sec-1 locus on 1RS. Twelve ω-secalin genes were arranged in a head-to-tail fashion, separated by 8.2-21.6 kb spacers on the contig, whereas six other ω-secalin genes were arranged head-to-tail, separated by 8.2-8.4 kb of spacers on clone BAC125. The 18 ω-secalin genes can be classified into six types among which eight ω-secalin genes were expressed during seed development. The ω-secalin genes with the 1074-bp open reading frame (ORF) represented the main population. Except for two pseudogenes, the N-terminal of the ω-secalin gene was conserved, whereas variations in the C-terminal led to a change in ORF length. The spacers can be sorted into two classes. Class-1 spacers contained conserved and non-conservative sequences. Conclusion: The ω-secalin gene family consisted of at least 18 members in the 1BL/1RS translocation line cv. Shimai 15. Eight ω-secalin genes were expressed during seed development. Eighteen members may originate from a progenitor with a 1,074-bp ORF. The spacers differed in length and sequence conservation. |
| author |
Li,Meng Jun Li,Ya Qing Zhang,Nan Shi,Zhan Liang |
| author_facet |
Li,Meng Jun Li,Ya Qing Zhang,Nan Shi,Zhan Liang |
| author_sort |
Li,Meng Jun |
| title |
Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| title_short |
Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| title_full |
Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| title_fullStr |
Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| title_full_unstemmed |
Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing |
| title_sort |
cloning of the ω-secalin gene family in a wheat 1bl/1rs translocation line using bac clone sequencing |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2016 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300010 |
| work_keys_str_mv |
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| _version_ |
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