Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis

Background: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 waste...

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Autores principales:	Parra,Bárbara, Robeson,James
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Bacteriophage Polyvalent Salmonella enterica serovar Choleraesuis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300011
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spelling	oai:scielo:S0717-345820160003000112016-07-06Selection of polyvalent bacteriophages infecting Salmonella entérica serovar CholeraesuisParra,BárbaraRobeson,James Bacteriophage Polyvalent Salmonella enterica serovar Choleraesuis Background: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 wastewater samples, collected at different sites in the V Region in Chile, we selected three bacteriophages (FC, FP, and FQ) capable of productively infecting Salmonella enterica serovar Choleraesuis. By transmission electron microscopy (TEM) observation, the bacteriophages were found to belong to the order Caudoviridae. Molecular analyses indicated that FC, FP, and FQ contained double-stranded DNA genomes, of sizes similar to bacteriophage P22, and distinct recognition sites for the restriction endonucleases HaeIII and HindIII. Assays of host range revealed that the bacteriophages were polyvalent and thus capable of infecting different strains of Escherichia coli and other serovars of Salmonella. Conclusion: We have isolated new bacteriophages of the serovar Choleraesuis with various potential applications in relation to this pathogenic bacterium.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.3 20162016-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300011en10.1016/j.ejbt.2016.01.008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacteriophage Polyvalent Salmonella enterica serovar Choleraesuis
spellingShingle	Bacteriophage Polyvalent Salmonella enterica serovar Choleraesuis Parra,Bárbara Robeson,James Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
description	Background: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 wastewater samples, collected at different sites in the V Region in Chile, we selected three bacteriophages (FC, FP, and FQ) capable of productively infecting Salmonella enterica serovar Choleraesuis. By transmission electron microscopy (TEM) observation, the bacteriophages were found to belong to the order Caudoviridae. Molecular analyses indicated that FC, FP, and FQ contained double-stranded DNA genomes, of sizes similar to bacteriophage P22, and distinct recognition sites for the restriction endonucleases HaeIII and HindIII. Assays of host range revealed that the bacteriophages were polyvalent and thus capable of infecting different strains of Escherichia coli and other serovars of Salmonella. Conclusion: We have isolated new bacteriophages of the serovar Choleraesuis with various potential applications in relation to this pathogenic bacterium.
author	Parra,Bárbara Robeson,James
author_facet	Parra,Bárbara Robeson,James
author_sort	Parra,Bárbara
title	Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
title_short	Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
title_full	Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
title_fullStr	Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
title_full_unstemmed	Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
title_sort	selection of polyvalent bacteriophages infecting salmonella entérica serovar choleraesuis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300011
work_keys_str_mv	AT parrabarbara selectionofpolyvalentbacteriophagesinfectingsalmonellaentericaserovarcholeraesuis AT robesonjames selectionofpolyvalentbacteriophagesinfectingsalmonellaentericaserovarcholeraesuis
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Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis

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