Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI

Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer units. This study aims to optimize the cellulase-free xylanase production and cell...

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Autores principales:	Khusro,Ameer, Kannan Kaliyan,Barathi, Abdullah Al-Dhabi,Naif, Valan Arasu,Mariadhas, Agastian,Paul
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Bacillus tequilensis Biomass Central composite design RSM Xylanase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400003
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spelling	oai:scielo:S0717-345820160004000032016-09-13Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATIKhusro,AmeerKannan Kaliyan,BarathiAbdullah Al-Dhabi,NaifValan Arasu,MariadhasAgastian,Paul Bacillus tequilensis Biomass Central composite design RSM Xylanase Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer units. This study aims to optimize the cellulase-free xylanase production and cell biomass of Bacillus tequilensis strain ARMATI using response surface methodology (RSM). Results: Statistical screening of medium constituents and the physical factors affecting xylanase and biomass yield of the isolate were optimized by RSM using central composite design at N = 30, namely 30 experimental runs with 4 independent variables. The central composite design showed 3.7 fold and 1.5 fold increased xylanase production and biomass yield of the isolate respectively compared to 'one factor at a time approach',inthe presence of the basal medium containing birchwood xylan (1.5% w/v) and yeast extract (1% w/v), incubated at 40°C for 24 h. Analysis of variance (ANOVA) revealed high coefficient of determination (R2)of0.9978 and 0.9906 for the respective responses at significant level (p < 0.05). The crude xylanase obtained from the isolate showed stability at high temperature (60°C) and alkaline condition (pH 9) up to 4 h of incubation. Conclusions: The cellulase-free xylanase showed an alkali-tolerant and thermo-stable property with potentially applicable nature at industrial scale. This statistical approach established a major contribution in enzyme production from the isolate by optimizing independent factors and represents a first reference on the enhanced production of thermo-alkali stable cellulase-free xylanase from B. tequilensis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.4 20162016-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400003en10.1016/j.ejbt.2016.04.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus tequilensis Biomass Central composite design RSM Xylanase
spellingShingle	Bacillus tequilensis Biomass Central composite design RSM Xylanase Khusro,Ameer Kannan Kaliyan,Barathi Abdullah Al-Dhabi,Naif Valan Arasu,Mariadhas Agastian,Paul Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
description	Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer units. This study aims to optimize the cellulase-free xylanase production and cell biomass of Bacillus tequilensis strain ARMATI using response surface methodology (RSM). Results: Statistical screening of medium constituents and the physical factors affecting xylanase and biomass yield of the isolate were optimized by RSM using central composite design at N = 30, namely 30 experimental runs with 4 independent variables. The central composite design showed 3.7 fold and 1.5 fold increased xylanase production and biomass yield of the isolate respectively compared to 'one factor at a time approach',inthe presence of the basal medium containing birchwood xylan (1.5% w/v) and yeast extract (1% w/v), incubated at 40°C for 24 h. Analysis of variance (ANOVA) revealed high coefficient of determination (R2)of0.9978 and 0.9906 for the respective responses at significant level (p < 0.05). The crude xylanase obtained from the isolate showed stability at high temperature (60°C) and alkaline condition (pH 9) up to 4 h of incubation. Conclusions: The cellulase-free xylanase showed an alkali-tolerant and thermo-stable property with potentially applicable nature at industrial scale. This statistical approach established a major contribution in enzyme production from the isolate by optimizing independent factors and represents a first reference on the enhanced production of thermo-alkali stable cellulase-free xylanase from B. tequilensis.
author	Khusro,Ameer Kannan Kaliyan,Barathi Abdullah Al-Dhabi,Naif Valan Arasu,Mariadhas Agastian,Paul
author_facet	Khusro,Ameer Kannan Kaliyan,Barathi Abdullah Al-Dhabi,Naif Valan Arasu,Mariadhas Agastian,Paul
author_sort	Khusro,Ameer
title	Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
title_short	Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
title_full	Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
title_fullStr	Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
title_full_unstemmed	Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
title_sort	statistical optimization of thermo-alkali stable xylanase production from bacillus tequilensis strain armati
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400003
work_keys_str_mv	AT khusroameer statisticaloptimizationofthermoalkalistablexylanaseproductionfrombacillustequilensisstrainarmati AT kannankaliyanbarathi statisticaloptimizationofthermoalkalistablexylanaseproductionfrombacillustequilensisstrainarmati AT abdullahaldhabinaif statisticaloptimizationofthermoalkalistablexylanaseproductionfrombacillustequilensisstrainarmati AT valanarasumariadhas statisticaloptimizationofthermoalkalistablexylanaseproductionfrombacillustequilensisstrainarmati AT agastianpaul statisticaloptimizationofthermoalkalistablexylanaseproductionfrombacillustequilensisstrainarmati
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Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI

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