Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco

Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ghaffar Shahriari,Amir, Bagheri,Abdolreza, Bassami,Mohammad Reza, Malekzadeh-Shafaroudi,Saeid, Afsharifar,Alireza, Niazi,Ali
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Agrobacterium tumefaciens ELISA Recombinant vaccine Transgenic plants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582016000400006
record_format	dspace
spelling	oai:scielo:S0717-345820160004000062016-09-13Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobaccoGhaffar Shahriari,AmirBagheri,AbdolrezaBassami,Mohammad RezaMalekzadeh-Shafaroudi,SaeidAfsharifar,AlirezaNiazi,Ali Agrobacterium tumefaciens ELISA Recombinant vaccine Transgenic plants Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Integration of the foreign gene in plant genome was confirmed by PCR. Expression of foreign gene was analyzed at transcription level by RT-PCR and at translation level by means of dot blotting and ELISA. All analyses confirmed the expression of recombinant protein. Conclusion: Developments in genetic engineering have led to plant-based systems for recombinant vaccine production. In this research, tobacco plant was used to express F and HN epitopes of NDV. Our results indicate that for the production of recombinant vaccine, it is a novel strategy to use concatenated epitopes without their genetic fusion onto larger scaffold structure such as viral coat protein.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.4 20162016-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006en10.1016/j.ejbt.2016.05.003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Agrobacterium tumefaciens ELISA Recombinant vaccine Transgenic plants
spellingShingle	Agrobacterium tumefaciens ELISA Recombinant vaccine Transgenic plants Ghaffar Shahriari,Amir Bagheri,Abdolreza Bassami,Mohammad Reza Malekzadeh-Shafaroudi,Saeid Afsharifar,Alireza Niazi,Ali Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
description	Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant expression binary vector pBI121, containing the genes encoding Hemagglutinin-Neuraminidase (HN) and Fusion (F) epitopes of Newcastle Disease Virus (NDV) under the control of CaMV35S promoter and NOS terminator was constructed and introduced into the tobacco ( Nicotiana tabacum) plant by Agrobacterium-mediated transformation. Results: Putative transgenic plants were screened in a selection medium containing 50 mg/L kanamycin and 30 mg/L meropenem. Integration of the foreign gene in plant genome was confirmed by PCR. Expression of foreign gene was analyzed at transcription level by RT-PCR and at translation level by means of dot blotting and ELISA. All analyses confirmed the expression of recombinant protein. Conclusion: Developments in genetic engineering have led to plant-based systems for recombinant vaccine production. In this research, tobacco plant was used to express F and HN epitopes of NDV. Our results indicate that for the production of recombinant vaccine, it is a novel strategy to use concatenated epitopes without their genetic fusion onto larger scaffold structure such as viral coat protein.
author	Ghaffar Shahriari,Amir Bagheri,Abdolreza Bassami,Mohammad Reza Malekzadeh-Shafaroudi,Saeid Afsharifar,Alireza Niazi,Ali
author_facet	Ghaffar Shahriari,Amir Bagheri,Abdolreza Bassami,Mohammad Reza Malekzadeh-Shafaroudi,Saeid Afsharifar,Alireza Niazi,Ali
author_sort	Ghaffar Shahriari,Amir
title	Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
title_short	Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
title_full	Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
title_fullStr	Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
title_full_unstemmed	Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco
title_sort	expression of hemagglutinin-neuraminidase and fusion epitopes of newcastle disease virus in transgenic tobacco
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400006
work_keys_str_mv	AT ghaffarshahriariamir expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco AT bagheriabdolreza expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco AT bassamimohammadreza expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco AT malekzadehshafaroudisaeid expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco AT afsharifaralireza expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco AT niaziali expressionofhemagglutininneuraminidaseandfusionepitopesofnewcastlediseasevirusintransgenictobacco
_version_	1718441931439079424

Expression of Hemagglutinin-Neuraminidase and fusion epitopes of Newcastle Disease Virus in transgenic tobacco

Ejemplares similares