Establishment of a HEK293T cell line able to site-specifically integrate and stably express GDNF by rAAV-2 vector

Establishment of a HEK293T cell line able to site-specifically integrate and stably express GDNF by rAAV-2 vector

Background: Using recombinant adeno-associated virus 2 (rAAV-2), we attempted to establish a HEK293T cell line that is able to site-specifically integrate and stably express glial cell line-derived neurotrophic factor (GDNF). Results:Recombinant vector with enhanced green fluorescent protein (EGFP)...

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Autores principales: Zhang,Jinju, Zhang,Yun, Liu,Xiaomei, Xiang,Jingjing, Zhang,Chun
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2016
Materias:
Glial cell line-derived neurotrophic factor
Multiplicity of infection
Recombinant adeno-associated virus 2
Site-specific integration
Transduction
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400011
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