Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49

The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and...

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Autores principales:	Xu,Ji-Fei, Mi,Yuan-Ting, Ren,Nan-Qi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Acetate Biohydrogen Buffering action Ethanoligenens harbinense phosphate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002
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spelling	oai:scielo:S0717-345820160005000022016-10-26Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49Xu,Ji-FeiMi,Yuan-TingRen,Nan-Qi Acetate Biohydrogen Buffering action Ethanoligenens harbinense phosphate The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH. At buffer concentrations 100-250 mM, the maximum hydrogen production was barely suppressed, and the lag phase extended past 7 h. Therefore, although acetate inhibits hydrogen production, using acetate as a buffer (like phosphate) effectively prevented pH drops and increased substrate consumption, enhancing hydrogen production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.5 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002en10.1016/j.ejbt.2016.07.002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Acetate Biohydrogen Buffering action Ethanoligenens harbinense phosphate
spellingShingle	Acetate Biohydrogen Buffering action Ethanoligenens harbinense phosphate Xu,Ji-Fei Mi,Yuan-Ting Ren,Nan-Qi Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
description	The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH. At buffer concentrations 100-250 mM, the maximum hydrogen production was barely suppressed, and the lag phase extended past 7 h. Therefore, although acetate inhibits hydrogen production, using acetate as a buffer (like phosphate) effectively prevented pH drops and increased substrate consumption, enhancing hydrogen production.
author	Xu,Ji-Fei Mi,Yuan-Ting Ren,Nan-Qi
author_facet	Xu,Ji-Fei Mi,Yuan-Ting Ren,Nan-Qi
author_sort	Xu,Ji-Fei
title	Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
title_short	Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
title_full	Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
title_fullStr	Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
title_full_unstemmed	Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49
title_sort	buffering action of acetate on hydrogen production by ethanoligenens harbinense b49
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002
work_keys_str_mv	AT xujifei bufferingactionofacetateonhydrogenproductionbyethanoligenensharbinenseb49 AT miyuanting bufferingactionofacetateonhydrogenproductionbyethanoligenensharbinenseb49 AT rennanqi bufferingactionofacetateonhydrogenproductionbyethanoligenensharbinenseb49
_version_	1718441933850804224

Buffering action of acetate on hydrogen production by Ethanoligenens harbinense B49

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